沙田柚自交不亲和花柱糖蛋白产生的时空关系

The time and space relations on the glycoprotein forming from self-incompatibility style of shatinyu

用辣根过氧化物酶标 Con A电镜细胞化学方法研究沙田柚不同发育时期的花柱通道细胞中糖蛋白的合成 ,分布和运输途径的动态变化。四分体期至开花期 ,在沙田柚花柱通道细胞中 ,内质网 (ER)合成糖蛋白呈颗粒状定位于核膜腔 ,内质网膜腔 ,质膜与细胞壁之间电子透明层中。授粉后 1～ 3d,内质网结构形态改变 ,由完整圆形变为开放 ,并囊泡化 ,大量线粒体集中于囊泡区 ,形成类似于哺乳动物腺外分泌细胞的高尔基区 ,内质网合成的糖蛋白在高尔基区膜囊中经糖基转移酶作用进一步浓缩凝结 ,形成与识别有关的糖蛋白 ,经高尔基小泡运送至通道细胞外壁。讨论了定位于通道细胞外壁的糖蛋白在沙田柚配子体型不亲和识别中的作用及与识别部位的关系。

Using Concanavalin A Horseradish peroxidase conjugates, a cytochemical method, the dynamic changes on synthesis, distribution and transport ways of the glycoproteins from the stylar passage cell of shatinyu( Citrus grandis var. shatinyu Hort.) in the distinct developing stages were researched. The glycoproteins synthesized in the endoplasm reticulum (ER) of the passage cell of shatinyu from the tetrad stage to the flowering stage showed granular shape. They were located in the cavities of nuclear membrane and ER membrane, and the electronic transpatent layer between plasmolemma and cell wall. In the first three day after pollinating, ER altered its shape, and its spiral was united and became vesiculation. A vast amount of mitochondriums concentrated in the vesicle zone and formed a Golgi zone similar to secretory cell outside the gland of mammal. The glycoproteins were enriched and coagulated continuously in the vesicle of Golgi zone by glycosyl transferases and formed the discernible glycoproteins. The glycoproteins were transported to the outer wall of passage cell through the Golgi vesicle. The role of glycoproteins on the discernment of gametophyte self incompatibility of shatinyu and the relations of the glycoproteins with discerning sites were discussed.