枸杞髓组织培养中体细胞胚胎发生与过氧化物酶同工酶分析

Observation of somatic embryogenesis and analyses of peroxidase isozymes in culture of the pith of Lycium barbarum L.

枸杞髓组织在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L培养基上诱导愈伤组织发生。在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L+CH50 0 mg/ L培养基上继代培养 ,再转入 MS+6 - BA2 mg/L +NAA 0 .5mg/ L的分化培养基上进行分化培养。显微观察表明 ,在培养过程中愈伤组织细胞由非胚性细胞转变为胚性细胞 ,直至发育成体细胞胚胎和完整植株 ;电泳结果显示 ,体细胞胚胎发生的各阶段 ,其过氧化物酶同工酶发生相应的变化。

WT5”BZ] Callus was induced on MS medium supplemented with 0 1 mg/L 6 BA and 0 5 mg/L NAA, from pith of Lycium barbarum L. and subcultured on MS medium supplemented with 0 1 mg/L 6 BA, 0 5 mg/L NAA and 500 mg/L CH. Swell pieces of calli were observed under the microscope and it was revealed that the nonembroynic cells of the callus had changed to embroynic cells from which plant was developed through somatic embryogenesis on the MS culture medium supplemented with 2 mg/L 6 BA and 0 5 mg/L NAA.Electrophoretic study of peroxidase isozymes had demonstrated corresponding changes during the stages of somatic embryogensis.