黄萎菌诱导棉花活性氧及保护酶系的变化研究

Changes of Reactive Oxygen and Protective Enzymes Activities in Cotton Challenged by Verticillium dahliae

以棉花耐黄萎病品种中棉41和感黄萎病品种冀棉11为材料,通过测定黄萎菌诱导前后活性氧积累及保护酶系活性的变化,研究其与棉花抗性的关系。结果表明:在接种黄萎菌后36h,2个品种的棉花叶片O2-产生速率均达到高峰,而中棉41O2-产生速率明显高于冀棉11;同时,叶片内丙二醛(MDA)的含量也均达到一个高峰,中棉41中MDA含量高于冀棉11;在接种后24h和36h,冀棉11和中棉41棉花叶片中超氧化物歧化酶(SOD)活性分别达到一个高峰,且冀棉11变化幅度大于中棉41;对于过氧化物酶(POD)的活性,在接种后24h,2个品种均达到一个高峰,但是耐病品种中POD活性增加幅度不及感病品种。在棉花与黄萎病菌互作的过程中,活性氧和保护酶系参与不同的抗性反应途径。

Changes of reactive oxygen and protective enzyme system in different cotton varieties,Zhongmian 41（tolerant variety） and Jimian 11（susceptible variety）,induced by V.dahliae were studied.It was shown that malondialdehyde（MDA） content of cotton leaves reached a peak after 36 h induced by V.dahliae,which in Zhongmian 41 was higher than in Jimian 11;meanwhile,the cotton leaf O-2 production rate of two varieties reached a peak,which in Zhongmian 41 was obviously higher than Jimina 11.At 24 h after inoculation,the cotton leaf superoxide dismutase（SOD） activity of Jimian 11 reached a peak,and its variance amplitude was bigger than that of Zhongmian 41,for which the SOD activity reached a peak after 36 h.At 24 h after inoculation,the peroxidase（POD） activity of both varieties reached a peak,but the increase amplitude in thedisease-tolerant variety was less than in the susceptible variety.During the interaction of cotton with V.dahliae,reactive oxygen and protective enzyme system were involved in different resis-tance-response pathway.