新疆大豆疫霉菌的毒力组成研究

Study on Virulence Structure of Phytophthora sojae in Xinjiang

为明确大豆疫霉菌在新疆的分布和新疆大豆疫霉菌的毒力组成,采用大豆叶碟诱捕法从新疆大豆田土壤中分离大豆疫霉菌,并采用幼苗下胚轴伤口接种法鉴定大豆疫霉菌的毒力。结果共分离到26个大豆疫霉菌株,毒力测定鉴定出20个不同的毒力型,说明新疆的大豆疫霉菌表现出丰富的毒力多样性。新疆大豆疫霉菌对抗病基因Rps1a,Rps1c和Rps1k的毒力频率均为0,因此,可应用这3个抗病基因对新疆大豆疫霉根腐病进行有效控制。

Phytophthora sojae was isolated from soil samples collected in Xinjiang by the soybean leaf-disc baiting method,and then their pathotypes were characterized using the hypocotyl slitinoculation method.A total of 26 P.sojae isolates were obtained and 20 pathotypes were identified from these isolates.The result indicated that virulence diversity of P.sojae was abundant in Xinjiang.All the isolates were avirulent to cultivars with single resistance genes Rps1a,Rps1c,and Rps1k,so these resistance genes had a high application value in better control of Phytophthora root and stem rot.