摘要
为了优化优无核葡萄的扦插技术,为其大面积生产提供科学育苗指导,研究了不同质量浓度国光生根粉对电热温床培养优无核葡萄插穗地下部分愈伤组织诱导率、生根率及其地上部分萌芽率、成活率和新梢生长势的影响。结果表明,插穗生长15d,清水对照的优无核葡萄插穗发芽率最高,达60%;国光生根粉质量浓度为2.5、3.7g/L时,发芽率次之,为35%。与清水对照相比,不同质量浓度的国光生根粉对葡萄插穗萌芽有极显著的抑制作用(P<0.01)。国光生根粉质量浓度为3.7g/L时,插穗愈伤组织诱导率、生根率均最高,分别为100%、40%,比对照提高35、40个百分点。与清水对照相比,不同质量浓度的国光生根粉对葡萄插穗愈伤组织形成和生根有极显著的促进作用(P<0.01)。插穗生长75d,国光生根粉质量浓度为3.7g/L时成活率达到最高,为95%,与其他处理相比差异达极显著水平(P<0.01)。插穗生长75d,各处理之间插穗新梢长度和新梢叶片数没有显著差异。表明国光生根粉3.7g/L处理对插穗萌芽抑制作用最小,而对插穗的生根以及成活的促进作用最大。因此,建议在优无核葡萄苗木生产中采用国光生根粉3.7g/L处理,以提高苗木成活率及苗木长势。
In order to improve cutting technology and provide seedling guidance for production of Superior Seedless,effect of different mass concentration of the Guoguang rooting powder on callus induction,rooting rate,germination rate,cutting survival rate and new shoot growth potential of Superior Seedless grape cutting were studied.The results showed that 15 d later the cutting came to grow,and the cutting germination rate of CK was up to 60%.When the Guoguang rooting powder's mass concentration was 2.5 g/L and 3.7 g/L,the cutting germination rate was 35%.Different mass concentration of the Guoguang rooting powder could inhibit germination rate of cutting significantly(P0.01) compared with CK.When the Guoguang rooting powder's mass concentration was 3.7 g/L,callus induction rate and rooting rate was up to 100% and 40%,increasing 35 and 40 percentage points compared with CK.Different mass concentration of the Guoguang rooting powder promoted callus induction rate and rooting rate of cutting significantly(P0.01) compared with CK.After 75 d the cutting came to grow,with survival rate up to 95% when it was 3.7 g/L,and the differences reached significant level(P0.01) compared with other treatments.Different treatments had no inhibition effect on new branch length and leaf number significantly.The treatment of 3.7 g/L had minimal inhibition effect on cutting germination and maximal promotion on rooting and survival.It suggested that Superior Seedless grape seedlings were treated with the Guoguang rooting powder of 3.7 g/L in order to increase cutting survival rate in nursery seedling production.
出处
《河南农业科学》
CSCD
北大核心
2012年第7期128-130,共3页
Journal of Henan Agricultural Sciences
基金
国家科技部星火计划项目(2007EA891008)
关键词
优无核
葡萄
育苗
国光生根粉
愈伤组织
扦插效应
Superior Seedless
grape
culture of seedlings
Guoguang rooting powder
callus
cutting effect