再生蛋白Ⅰ在慢性萎缩性胃炎大鼠中的表达研究

The study of regeneration gene Ⅰ in an atrophic gastritis rat model

目的建立慢性萎缩性胃炎的Wister大鼠模型,检测在此模型大鼠的胃黏膜中再生基因Ⅰ(RegⅠ)的表达情况,以了解RegⅠ在慢性萎缩性胃炎大鼠中的作用。方法以2%的水杨酸钠、30%的乙醇的混合液及20 mmol/L去氧胆酸钠间日灌胃,0.1%氨水饮用并结合饥饿饱餐因素构建慢性萎缩性胃炎的大鼠模型,荧光定量PCR检测RegⅠ及其受体Reg-R的mRNA表达,Westen blot检测RegⅠ的蛋白水平表达;用免疫组化染色检测大鼠胃黏膜增殖细胞核抗原(PCNA)的表达。结果成功建立了慢性萎缩性胃炎的Wister大鼠模型,荧光定量PCR显示RegⅠ的mRNA在两组间并无显著差异,但模型组大鼠胃黏膜中RegⅠ的蛋白较对照组升高,且RegⅠ-R的mRNA水平明显升高(P<0.05);而其腺体中PCNA的阳性表达也较对照组高。结论在此慢性萎缩性胃炎大鼠模型中存在RegⅠ的高表达,同时其胃黏膜腺体细胞增殖旺盛,提示RegⅠ在慢性萎缩性胃炎大鼠中可能起到促进腺体增生修复黏膜的作用。

Objective We established an chronic atrophic gastritis （AG） rat model to detect the expression of regeneration gene I （RegI） and the proliferation of mucus gland, Methods The AG rat model was established by administration intermixture of 2% sodium salicylate and 30% alcohol, 20mmol/L sodium desoxycholate, 0. 1% ammonia in stead of water, combining irregular feeding. Regl and RegR mRNA expression of gastric mucosa were estimated by real - time quantitative PCR （ Q - PCR） and western blot was used to detect the RegI protein expression. The cell proliferation in gastric glands was estimated by immunohistochemistry staining of PCNA. Results We successfully established an AG rat mod- el. The RegI protein expression was increased in gastritis tissue in rats. The Q - PCR showed there was no significant difference in RegI mRNA ex- pression between two groups （ P 〉 0.05）, however, tile Reg - R was significantly elevated in model rats （ P 〈 0.05）. The immunohistochemistry staining of PCNA suggested cell proliferation in gastric glands was increased in the model rats. Conclusion RegI is overexpressed in the chronic AG rat model, combined with the cell proliferation of gastric glands. It suggests that RegI may increase the damaged mucus glands repair in AG rat.