p38、NF-кB、IL-6在少肌症发生及其运动性缓解中的作用

Effect of p38,NF-кB and IL-6 on pathogenesis of sarcopenia and it's exercise-induced improvement

目的:研究p38、NF-кB、IL-6在少肌症发生及运动缓解少肌症中的作用。方法:24只雄性SD大鼠分为3组,C组(青年安静组)、S组(40 d D-半乳糖注射致衰组)、E组(40 d D-半乳糖注射+6 w跑台运动组)。检测C、S、E组大鼠腓肠肌的质量、Phospho-p38、p38、Phospho-p65、p65、IL-6 mRNA、MRFs(MyoD、MyoG、Myf-5、MRF4)mRNA。结果:与C组相比,S组腓肠肌/体重、Phospho-p65、IL-6 mRNA降低,Phospho-p38、MyoD mRNA、MyoGmRNA、Myf-5 mRNA上升,与S组相比,E组腓肠肌/体重、Phospho-p38、Phospho-p65、IL-6 mRNA、MyoD mRNA、MyoG mRNA、MRF4 mRNA升高;MyoD mRNA/MyoG mRNA比值为S组低于C组,E组高于S组;C组、S组、E组p38及p65均无显著性差异。结论:NF-кB与IL-6(或以NF-кB/IL-6形式)介导、而p38、MyoD、MyoG、Myf-5(或以p38/MyoD、MyoG、Myf-5形式)拮抗衰老引起肌肉流失(少肌症);p38、NF-кB、IL-6、MyoD、MyoG、MRF4参与了运动对少肌症的缓解,该过程可能以p38、NF-кB、IL-6(或以p38/NF-кB/IL-6形式)/MyoD、MyoG、MRF4通路形式进行;衰老诱导肌肉快-慢转化,运动抑制了该转化趋势;p38、p65表达对运动、衰老不敏感。

Objectvie ： Explorer the function of p38, NF-κ B and IL - 6 in pathogenesis of sarcope- nia and its exercise - induced improvement. Methods ： 24 male SD rats were divided into 3 groups ： C group（ quiet young group）, S group（40 day D-Glactose injection -induced senecence） , E group （40 days D -Glactose injection- induced senecence ＋ 6weeks exercise on treadmill）. It detectedthe body weight, gastrocnemius weight, Phospho -p38, p38, Phospho -p65, p65, IL -6 mRNA, MRFs（ MyoD, MyoG, Myf - 5, MRF4） mRNA. Results ： There was lower ratio of gastrocnemius weight to body weight, Phospho- p65, IL-6 mRNA, higher Phospho- p38, MyoD mRNA, MyoG mRNA, Myf -5 mRNA in S group than C group. There was higher ratio of gastrocnemius weight to body weight, Phospho - p38, Phospho - p65, IL - 6 mRNA, MyoD mRNA, MyoG mRNA, MRF4 mRNA in E group than S group. There is lower or higher ratio of MyoD mRNA to MyoG mRNA in S group than C group or than E group. No change of p38 or p65 in S or E group compared with C group. Conclutions ： NF - KB and IL - 6 meditated but p38, MyoD, MyoG, Myf - 5 resisted sar- eopenia which may be meditated by NF-κB, IL- 6 and resisted by p38/MyoD, MyoG, Myf-5; p38, NF - KB, IL - 6, MyoD, MyoG, MRF4 contributed to exercise - induced improvement of sar- copenia which may be meditated by p38, NF -κB, IL - 6 （ or p38/NF -κB/IL - 6）/ MyoD, MyoG, MRF4; Senescence induced ＂fast to slow＂ type transformation in skeletal muscle and the transformation was inhibited by exercise. Both p38 and p65 are insensitive to senescence and exercise.