人类乳头瘤病毒与宫颈癌关系的研究

CORRELATION OF HUMAN PAPILLOMA VIRUS(HPV)AND CERVICAL CANCER

1987～1988年,我们采取116例宫颈癌病人和36例无宫颈疾病妇女的宫颈组织,以(α-32P)-dCTP标记的HPV 16 DNA BamHI酶解片段为探针,用Southern blot核酸杂交技术进行了BPV 16 DNA相关序列检测。本文就检测结果,结合临床和流行病学,探讨HPV与宫颈癌的关系。 材料和方法 一、组织来源 宫颈组织标本采自山东医科大学附属医院,山东省肿瘤防治院及山东省千佛山医院妇科门诊和住院病人,均经病理组织学确诊。

With the probe (α-^(32)P)-dCTP HPV16 DNA fragment cleaving with BamHI, sam-ples of cervical tissue from Shandong Province were tested by Southern blot hybridiza-tion technique. The total positive rate of HPV16 DNA related sequences in 116 caseswith cervical cancer was 50.9%, much higher than that in 36 cases with non-cervicalcancer disease(5.6%). There is a strong correlation between the HPV and cervicalcancer (OR=17.60, P<0.001). For the first time in Shandong Province, HPV33 DNAsequences were found in four and HPV31 DNA sequences in one cervical carcinomasamples. The result indicated that there could be an etiological relationship betweenHPVinfection and cervical cancer.