鸭乙型肝炎病毒感染对鸭肝中黄曲霉毒素-DNA加成物形成的影响

EFFECT OF DUCK HEPATITIS B VIRUS INFECTION ON AFLATOXIN-DNA-ADDUCTS FORMATION IN DUCK LIVER

人类肝癌的发生可能与乙型肝炎病毒（HBV）和黄曲霉毒素B1（AFB1）等致癌因素有关。有人推测HBV感染可能会导致肝微粒体酶对AFB1代谢模式的改变,从而增强AFB1的致癌效应,也有人认为HBV与AFB1在人类肝癌发生中可能具有协同作用。但迄今尚缺少直接的实验依据。鸭乙型肝炎病毒（DHBV）与HBV同属嗜肝DNA病毒科。鸭对AFB1非常敏感,因此家鸭是研究HBV与AFB1相互作用的良好动物模型。本文以DHBV感染及未感染的家鸭为材料,在给予AFB1后,比较两组鸭肝中形成黄曲霉毒素-DNA（aflatoxins-DNA）加成物的百分率,为阐明DHBV感染是否会增强AFB1的致癌效应提供实验依据。

The possibility of duck hepatitis B virus (DHBV) infection altering the metabolismof aflatoxin B_1(AFB_1) and enhancing the aflatoxin-DNA-adducts formation in duckliver was examined. AFB_1,was administered by oral intubation to ducks infected oruninfected with DHBV.After a single dose of AFB_1 the animals were sacrificed at 1,2, 4, 8, 12, 24, 48 and 72 hr respectively. Liver DNA adducts were examined by detecting[~3H] aflatoxin-DNA-adducts and HPLC analysis. AFB_1-N^7-Gua was the major DNAadducts found in duck liver at 1 and 2 hr postdosing and disappeared mostly at 24 hr.At 48 and 72 hr only AFB-1-FAPyr was detectable. There was no significant differencein total amount of DNA adducts formed in liver of DHBV-positive ducks as comperedwith DHBV-negative ones. The result showed that DHBV infection did not enhanceaflatoxin-DNA-adducts formation and aggravate the carcinogenicity of AFB_1.