胃癌单克隆抗体MG_7相关抗原的初步分析

PRELIMINARY ANALYSIS OF GASTRIC CANCER ASSOCIATED GLYCOLIPIDS ANTIGEN

国内外研究表明应用单克隆抗体技术可能建立一种特异、敏感、快速的胃癌诊断方法。近年来,第四军医大学西京医院建立了10余株抗不同组织学类型胃癌的克隆杂交株。ABC免疫组化技术及阳性扫描研究提示该组单克隆抗体有较大的应用价值。初步研究表明:抗胃癌单克隆抗体MG_7的相关抗原可能为中性糖脂。本室应用糖脂的免疫染色技术(Immunostaining)对MG_7的相关糖脂抗原作了初步分析。

The tumor associated glycolipids antigens were extracted from gastric canoer tissueand purified by using organic solvents and ion exchange, absorbtion chromatographymethods. These antigens were identified as neutral glycolipids by high performancethin layer chromatography. Comparing with standard neutral glycolipids pattern, theirRf values on HPTLC plate were below the Forssman antigen and had more than eightpolysaccharide residues. At least six components of them could be reacted with MG-monoclonal antibody specifically by immunostaining methods.