活血解毒方对糖尿病周围神经病变大鼠PKC及MDA的影响

Effect of "Huoxue Jiedu Prescription" on PKC and MDA in rats with diabetic neuropathy

目的观察活血解毒方对糖尿病大鼠周围神经病变的疗效及作用机制。方法将50只雄性SD大鼠分为正常组、模型组、甲钴胺组、活血解毒方不同剂量组。除正常组外,其余各组予链脲佐菌素腹腔注射(65μg/g)造模,并给予相应的干预措施,16周后观察大鼠血糖、热板潜伏期、神经传导速度、坐骨神经病理的变化情况,检测坐骨神经中蛋白激酶C(PKC)的表达及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量及总抗氧化能力。结果与正常组比较,模型组大鼠血糖显著升高,热板潜伏期延长,神经传导速度减慢,髓鞘松解,血清中SOD活性降低、MDA升高、总抗氧化能力减弱,坐骨神经内PKC表达增加,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,活血解毒方高、低剂量组热板潜伏期缩短,神经束膜整齐,PKC在坐骨神经内表达减少,血清中MDA降低,活血解毒方高剂量组神经传导速度增快,差异有统计学意义(P<0.05)。结论活血解毒方可改善糖尿病大鼠周围神经病变,其机制可能与抑制PKC及MDA有关。

Objective To observe the efficacy and mechanism of ＂Huoxue Jiedu Prescription＂ on neuropathy of diabetic rats, Methods The male SD rats were divided into normal group, model group, methyleobzlamin group and ＂Huoxue Jiedu Prescription＂ group. The rat models were established by streptozocin injecting intraperitoneally. The blood sugar, threshold of hot plate test, nerve conduction velocity and pathological changes were observed, and the expression of PKC, contents of SOD and MDA, and the total antioxidant capacity were detected. Results Compared with normal group, the blood sugar was increased, latency of hot plate was delayed, nerve conduction velocity was decreased, myelin sheaths was loose, the activity of SOD was decreased, the MDA was increased, total antioxi- dant capacity was reduced, expression of PKC was increased, with significant differences between the normal group and model group （ P 〈 0.05, P 〈 0.01 ）. Compared with model group, the latency of hot plate was shortened, the perineurium was in order, the expression of PKC was reduced, the MDA was lowered in large and small dose ＂Huoxue Jiedu Prescription＂ group, and the nerve conduction velocity was increased in large dose ＂Huoxue Jiedu Prescription＂ group, with a significant difference between model group and ＂Huoxue Jiedu Prescription＂ group （ P 〈 0.05 ）. Conclusion ＂Huoxue Jiedu Prescription＂ can improve the neuropathy in diabetic rats by inhibiting of PKC and MDA.