鸡血藤总黄酮对小鼠急性肝损伤的保护作用

Protective effects of suberect spatholobus total flavonoids on acute hepatic injury in mice

目的研究鸡血藤总黄酮对四氯化碳诱导小鼠肝损伤的保护作用。方法 60只雄性小鼠随机分为6组,每组10只。除正常组外,其余各组均腹腔注射0.2%的CCl4橄榄油溶液诱导小鼠急性肝损伤,测定血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)、肝组织超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量,HE染色观察肝组织病理学变化。结果鸡血藤总黄酮明显抑制CCl4致血清ALT、AST活性的升高和肝组织SOD、GSH-Px活性的降低及MDA水平的升高。肝组织形态学观察显示鸡血藤总黄酮能显著改善肝组织的病理变化。结论鸡血藤总黄酮对CCl4诱导小鼠急性肝损伤具有一定的保护作用。

Objective To investigate the protective effect of Suberect Spatholobus Total Flavonoids（SSTF） on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Methods Sixty male mice were randomly separated into six groups and each group had ten mice.Except for the normal group,all groups were injected intraperitoneally with 0.2 % CCl4 olive oil solution to induce acute liver injury.The activity of Alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in serum,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） activities and malondialdehyde（MDA） content in liver were determined.The hepatic histopathological changes were observed by HE staining.Results The results showed that treatment with SSTF significantly prevented the activities elevation of ALT,AST in serum and decrease of SOD,GSH-px in liver,and the content increase of MDA in liver.The observation of hepatic tissue pathological slices showed SSTF could significantly improve hepatic histopathological changes.Conclusion SSTF has Certain protective effect on acute Hepatic injury induced by CCl4 in mice.