拟南芥山奈酚糖苷对UV-B辐射的响应

Effect of UV-B radiation on kaempferol glycosides in Arabidopsis thaliana

以拟南芥(Arabidopsis thaliana)为材料,通过施加低强度(40μw.cm-2)和相对长时间(7 d)的UV-B辐射,研究拟南芥幼苗和成苗中三种山奈酚糖苷即山奈酚-3-O-鼠李葡萄糖基-7-O-鼠李糖苷(K1)、山奈酚-3-O-葡萄糖基-7-O-鼠李糖苷(K2)、山奈酚-3-O-鼠李糖基-7-O-鼠李糖苷(K3)含量及合成途径关键酶编码基因表达水平对UV-B辐射的响应。结果表明,拟南芥幼苗三种山奈酚糖苷含量均为成苗的10倍左右。经过7 d的UV-B辐射处理,拟南芥幼苗中山奈酚糖苷积累水平、合成途径关键酶编码基因chs和fls1的相对表达量均显著高于对照植株,山奈酚糖苷表现为响应UV-B辐射而积累。在拟南芥成苗和幼苗中三种山奈酚糖苷组合模式应对UV-B辐射的变化并不一致,这与其在防御机制中的不同角色有关。

The profiles of kaempferol glycosides,including kaempferol-3-O-rhamnosyl glucoside7-O-rhamnoside（K1）,kaempferol-3-O-glucoside-7-O-rhamnoside（K2） and kaempferol-3-O-rhamnoside7-O-rhamnoside（K3）,and the expression of related genes in response to UV-B radiation（40 μw·cm-2） for 7 d was analyzed in Arabidopsis thaliana.The results showed that the contents of three kaempferol glycosides in young seedlings were about 10 times of those in mature seedlings.All three kaempferol glycosides contents and the expression of kaempferol glycosides biosynthesis genes chs and fls1 in young seedlings were higher than those in control plants,and the kaempferol glycosides were accumulated in response to UV-B exposure for 7 d.The changes of different kaempferol glycosides profile in response to UV-B treatment were different between young and mature seedlings,which could be dued to different roles in defense mechanisms.