地毯草根际固氮菌的分离及鉴定

Isolation and identification of associative nitrogen-fixing bacteria in the rhizosphere of Axonopus compressus

结合气相色谱仪,利用乙炔还原等方法对地毯草（Axonopus compressus）根际固氮菌进行分离和鉴定。结果表明,从地毯草根际土壤中分离获得6株固氮菌株,菌株的固氮酶活性相差较大（92.5 C2H4~295.2 C2H4nmol.mL-1.h-1）,菌株hnN2及hnN6具有较高的固氮酶活性（〉200 C2H4nmol.mL-1.h-1）;菌株经鉴定分属Bacillus（2株）、Azotobacte（r 3株）和Pseudomona（s 1株）3个属。

This study isolates and identifies associative nitrogen-fixing bacteria from the rhizosphere of Axonopus compressus by acetylene reduction assay（ARA） combined with gas chromatography（GC）.Six strains were isolated,and showed that nitrogenase activity was large variation between strains（from 92.5 C2H4 to 295.2 C2H4nmol.mL-1.h-1）.Strains hnN2 and hnN6 showed higher nitrogenase activity（200 C2H4 nmol.mL-1.h-1）.All strains belonged to Bacillus（2 strains）,Azotobacter（3 strains） and Pseudomonas（1 strains）,respectively.