摘要
目的探讨大鼠继发性脑损伤(SBI)后海水浸泡对脑组织病理改变的影响。方法 163只SD大鼠随机分为正常对照(A组,5只)、SBI(B组3,2只)、SBI合并生理盐水浸泡(C组3,3只)及SBI合并海水浸泡(D组9,3只)4组。Marmarou弥漫性脑损伤模型基础上建立缺血性SBI合并海水浸泡动物模型。伤后13、、6、12、244、8h在挫伤区取材,测定脑组织含水量,并作HE染色,光镜下观察创伤脑组织的病理变化。结果 D组较之A组在伤后1h便出现脑组织含水量的明显变化(P<0.05)。脑组织含水量呈持续性升高,且各时相均显著高于B、C组(P<0.05)。HE染色光镜下观察见B、C组脑组织细胞呈海绵状改变;而D组脑组织损伤更严重,呈网格状改变。结论 SBI合并海水浸泡后脑水肿比单纯SBI严重,且伤情发展更迅速而持久。这种伤情变化与海水特有的损伤性因素密切相关。
Objective To study the pathological impact of seawater immersion on rat brain tissue with secondary brain insults(SBI).Methods Totally 163 male SD rats were randomly divided into four groups:control group(A,5 rats),SBI group(B,32 rats),SBI with saline immersion group(C,33 rats) and SBI with seawater immersion group(D,93 rats).On the basis of the Marmarou's rat diffuse brain injury model,the animal models of SBI with seawater immersion were established by bilateral common carotid artery ligation and persistent seawater infusion to cerebral tissues.Rats were killed and the contents of water were determined at 1,3,6,12,24 and 48 hours after SBI.The pathological changes in the brain tissues were observed by means of HE staining.Results Compared with group A,remarkable changes of brain water contents appeared at 1hour in group D(P0.05),whereas 3 hours in group B and C.Brain water contents of group D maintained a persistent increase which were significantly higher than those of group B and C at all the phases(P 0.05).Pathological changes by HE staining indicated that traumatic edema was aggravated as a result of seawater immersion in group D and presented grid change,different from the spongiform change of group B and C.Conclusion SBI with seawater immersion is more severe and more quickly and lastly than simple SBI.This variation feature is closely related to seawater's detrimental factors.
出处
《创伤外科杂志》
2012年第5期431-434,共4页
Journal of Traumatic Surgery
关键词
脑损伤
海水浸泡
脑组织
病理学
brain insults
seawater immersion
brain tissue
pathology