黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析

Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora ♀ and Larimichthys crocea ♂

开展了黄姑鱼♀与大黄鱼♂杂交试验,并对杂交F1初孵仔鱼进行了AFLP分析.黄姑鱼♀与大黄鱼♂杂交能够正常受精,杂交受精率为93.54%,受精卵正常发育、孵化,孵化率为86.23%,但仔鱼在开口后一周内全部死亡.利用8对选择性扩增引物组合对亲本和杂交后代(家系)初孵仔鱼进行AFLP分析,共得到456个条带,其中母本特异条带(Female Special Band,FSB)178条、父本特异条带(Male Special Band,MSB)187条、双亲共有条带(Mutual Band,MuB)91条.且其中84条(47.2%)FSB、89条(47.6%)MSB和75条(82.4%)MuB为全部杂交仔鱼共有,其余94条(52.8%)FSB、98条(52.4%)MSB和16条(17.6%)MuB在杂交后代中发生了分离.分离的FSB和MSB中分别有33.0%和28.6%表现为偏分离.AFLP分析结果表明,黄姑鱼♀与大黄鱼♂杂交的初孵仔鱼含有双亲的条带,是真正的杂交种,是构建大黄鱼与黄姑鱼遗传图谱以及研究杂种在细胞和分子水平隔离机制的适合材料.

Hybridization experiments between Nibea albiflora ♀ and Larimichthys crocea ♂ were carried out.The average fertilization rate and the hatching rate of the hybrid cross were 93.54% and 86.23%,respectively.However,the hybrids were all dead within one week after hatching.To elucidate the genetic composition of the hybrids,32 larvae from a hybrid family（NL） was investigated by using AFLP technology.Totally,456 AFLP bands were detected in parents and progenies,which included 178 female parent-specific bands（FSB）,187 male parent specific bands（MSB） and 91 mutual bands（MuB）.All of the fragments were detected in the hybrid progenies,including 84（47.2%）FSB,89（47.6%）MSB and 75（82.4%）MuB.MuB was found in all the progenies,while other bands were segregated in the progenies,with 33.0% of FSB and 28.6% of MSB distorted segregation.The AFLP results suggested that the larvae of N.albiflora ♀ × L.crocea ♂ were the true hybrids containing whole nuclear genome of both parents,so they are applicable to construct the genetic maps of female N.albiflora and that of male L.crocea.