卵巢肿瘤原位移植动物模型的建立

The Establishment of Animal Model of Orthotopically Transplanted Ovarian Carcinoma

目的:建立能够模拟临床肿瘤发展过程的卵巢癌动物模型。方法:将处于对数生长期的人卵巢癌细胞株SKOV3(2×106个/只)、A2780多水平细胞数(2×106个/只、5×106个/只、1×107个/只、1.5×107个/只和2×107个/只)裸鼠皮下注射,A2780(1.5×107个/只)腹腔注射。取SKOV3第3代连续传代皮下瘤组织进行裸鼠右侧卵巢包膜下移植。结果:卵巢癌细胞株SKOV3皮下注射、组织块原位移植成瘤率均100%。原位移植卵巢癌裸鼠6～8周时剖腹探查见局部形成较大包块,与周围组织发生粘连,腹腔暗红色血性积液伴脏器广泛性转移,淋巴结转移。卵巢癌A2780细胞株多水平细胞数皮下注射均未成瘤;腹腔注射8周时剖腹探查见腹腔内生成2～3 mL无色澄清腹水,双侧卵巢增大,脾脏明显增大,未能发现任何腹腔瘤块。结论:成功建立了临床转移模式的SKOV3卵巢癌肿瘤细胞原位移植动物模型;卵巢癌A2780细胞接种未能成瘤,可能是因其对宿主体内非特异性免疫反应较敏感。

Objective：To establish an animal model with ovarian carcinoma to simulate the clinical tumor development.Methods：Human ovarian carcinoma cell lines SKOV3 and multi-level A2780 in exponential phase of growth were inoculated subcutaneously in nude mice,A2780 cells were also inoculated intraperitoneally.The third generation of SKOV3 subcutaneous tumor was transplantated below the capsule of the right ovary.Results：Subcutaneous inoculations of human ovarian carcinoma SKOV3 cells and orthotopic transplantations of SKOV3 subcutaneous tumor both succeeded in all mice.Six to eight weeks after orthotopic transplantations,tumors invasion into surrounding tissues were observed,as well as dull red effusion with peritoneal metastasis and lymph node metastasis.The subcutaneous inoculation of multi-level cell lines A2780 all failed in tumor formation.Eight weeks after A2780 cells were injected in intraperitoneal cavity,2-3 mL colorless ascites appeared,ovarian and spleen grew obviously,but tumor was not detected.Conclusions：The SKOV3 orthotopically transplantated animal model was successfully established.The inoculation of A2780 cell lines failed in tumor formation,possibly due to their sensitivities to non-specific immunity in the host.