莪术油配伍椒目仁油对小鼠移植性肿瘤的抑制作用

The inhibition effect of transplanted mice tumor with curcuma oil compatibility from Seed oil

目的观察不同剂量莪术油配伍椒目仁油对小鼠移植性肿瘤S180的抑瘤作用,以及对人源性肝癌细胞株HepG2和大肠癌细胞株SW480的体外抑瘤作用。方法 72只二级昆明小鼠采用肿瘤移植法建立小鼠荷瘤动物模型;椒莪软胶囊灌胃给药,剂量分别为0.3、0.6、1.2 ml/kg;氟尿嘧啶22.5 mg/kg作为阳性对照药腹腔注射给药;连续给药15 d,观察药物对肿瘤生长的抑制作用。对人源性肝癌细胞株HepG2和大肠癌细胞株SW480进行传代培养后,制备不同剂量(椒莪软胶囊0.015 625、0.031 25、0.062 5、0.125、0.25、0.5、1、2、4 mg/ml)含药血清、氟尿嘧啶阳性药血清和肿瘤移植模型不用药对照血清,作用于人源性肝癌细胞株HepG2和大肠癌细胞株SW480,四甲基偶氮唑蓝比色法观察血清对两种细胞的抑制作用。椒莪软胶囊0.0、6.25、12.5、25.0μg/ml给药15 h后,经流式细胞仪测定对人源性肝癌细胞株HepG2细胞凋亡的影响。结果①椒莪软胶囊0.3、0.6、1.2 ml/kg肿瘤抑制率分别为19.73%、32.22%和46.68%。随着给药剂量的增加,坏死的肿瘤细胞所占比例也相应增加,生长活跃的肿瘤细胞逐渐减少。②抑制作用随剂量升高而作用增强,对人源性肝癌细胞株HepG2体外最低药物物浓度为0.062 5 mg/ml,对大肠癌细胞株SW480体外最低药物浓度为0.125 mg/ml。③椒莪软胶囊可明显促进人源性肝癌细胞株HepG2的细胞凋亡。结论椒莪软胶囊对小鼠移植性肿瘤有明显的抑制作用,对人源性肝癌细胞株HepG2和大肠癌细胞株SW480有明显的抑制作用。椒莪软胶囊在临床具有广阔的应用前景。

Objective To observe the inhibitory effect of different doses of Curcuma oil compatibility from Seed oil in mice transplanted tumor S180,as well as the in vitro inhibitory effect of human-derived HepG2 cells and colon carcinoma cell line SW480.Methods Seventy two Kunming mice with tumor xenografts established mouse tumor-bearing animal models.Jiaoe soft capsules administered orally at doses of 0.3,0.6 and 1.2 ml/kg,fluorouracil with 22.5 mg/kg abdominal injection as positive control,administration for 15 days to observe the inhibitory effect of drugs on tumor growth.The preparation of different doses of pepper to Curcuma soft capsules（0.015 625,0.031 25,0.062 5,0.125,0.25,0.5,1,2,4 mg/ml） containing fluorouracil-positive serum and tumor transplantation control group without serum,to observe role of serum inhibition in human derived hepatoma cell line HepG2 and colon cancer cell line SW480 by tetrazolium bromide colorimetric.Jiaoe soft capsules administered 15 h after the impact of human-derived HepG2 at doses of 0.0,6.25,12.5,25.0 μg/ml,cell apoptosis measured by flow cytometry.Results ①Jiaoe soft capsules at doses of 0.3,0.6,1.2 ml/kg,the tumor inhibition rates were 19.73%,32.22% and 46.68% respectively.With the increase of the dose,the proportion of the tumor necrosis increased and the active tumor cells decreased gradually.②The inhibition role increased with the doses raising.In vitro minimum drug concentration for by human-derived hepatoma cell line HepG2 was 0.062 5 mg/ml,for strains of SW480 colon cancer cells it was 0.125 mg/ml.③The Jiaoe soft capsule can significantly promote apoptosis of human-derived HepG2 cells.Conclusion Jiaoe soft capsule significantly inhibits the mice transplanted tumors by human-derived HepG2 cells and colon carcinoma cell line SW480.Jiaoe soft capsules in clinical practice has broad application prospects.