不同树龄厚朴生物活性成分的比较分析

Comparison analysis of bioactive compounds of Mignolia officinalis Rehd. et Wils from different growth ages

利用高效液相色谱法(HPLC)测定不同树龄厚朴中绿原酸、芦丁、金丝桃苷、槲皮苷、槲皮素、和厚朴酚和厚朴酚的含量。目的:为了比较分析同一来源不同树龄厚朴中7种活性物质含量变化,为最佳厚朴采集年龄提供参考。结果:绿原酸、金丝桃苷、槲皮素和厚朴酚在13年树龄时含量最高,分别达到0.28、0.033、0.018和5.90%;芦丁和槲皮苷在7年树龄时含量最高,其含量分别为0.34和0.52%;和厚朴酚和厚朴酚在10年树龄时其含量最高为2.40%。厚朴中的厚朴酚和和厚朴酚为主要成分在10年树龄和到26年树龄其成分的变化不大,10、13和26年树龄厚朴中7种活性物质总的含量分别为7.99、8.75和8.73%,总含量变化也不显著,因此,选10年的树为最佳厚朴采集年龄。

Chlorogenic acid, rutin, hyperoside, quercitrin, quercetin, magnolol and honokiol in Mignolia officinalis Rehd. et Wils from different growth ages were analyzed by high performance liquid chromatography with photodiode- array detection （HPLC-DAD）. The aim is to compare change of 7 bioactive compounds of from different growth ages, this will be demonstrated to be very useful in optimal M. officinalis collected. The results showed that the contents of chlorogenic acid, hyperoside, quercitrin and magnolol, which were highest at 13 year, were 0.28、0.033、0.018 and 5.90%, respective. The contents of rutin and quercetin , which were highest at 7 year, were 0.43 and 0.52%, respective. The content of honokiol which were highest at 10 year, were 2.40%. The content of magnolol and honokiol of M. officinalis from 10 to 26 year were not remarkably variational. The all contents of 7 compounds, which were not remarkably variational, were 7.99、8.75 and 8.73%,respectively. So optimal M. officinalis collected was 10 year ages.