肿瘤干细胞标志物ALDH1及CD133在乳腺癌中的表达及意义

Expressions and significances of tumor stem cells markers-ALDH1 and CD133 in breast cancer

目的:通过检测醛脱氢酶-1(ALDH1)及CD133的表达,探讨其作为乳腺癌干细胞标志物的敏感性和特异性。方法:应用免疫组织化学方法,检测乳腺癌干细胞标志物ALDH1及CD133在人乳腺癌组织中的表达。结果:ALDH1在乳腺癌中的表达阳性率为40.74%,ALDH1阳性染色主要分布于肿瘤细胞的胞浆,胞核不染色,阳性细胞散在分布于肿瘤组织中,部分区域可见阳性细胞密集分布现象。CD133阳性细胞表现为位于胞膜、胞浆上的棕褐色染色,胞核不染色。在27例乳腺浸润性导管癌中6例CD133呈阳性,阳性率为22.22%,并且随着乳腺癌组织学级别的增高其表达增高。结论:ALDH1及CD133联合应用可作为乳腺癌干细胞的特异性标志物。

Objective： To explore the sensitivities and specificities of aldehyde dehydrogenase -1 （ALDH1） and CD133 as tumor stem cells markers by detecting their expressions. Methods： Immunohistochemical method was used to detect the expressions of ALDH1 and CD133 in human breast cancer tissue. Results： The positive expression rate of ALDH1 in breast cancer was 40. 74% , the positive staining was mainly located in cytoplasm of breast cancer cells, the nucleus was not stained, the positive cells were distributed in breast cancer tissue diffusely and partial areas densely. The positive staining of CD133 were located in the cellular membrane and cytoplasm, the nucleus was not stained. Among 27 cases with invasive ductal carcinoma, 6 cases were found with positive CD133, the positive rate was 22. 22%, which in- creased with the increase of histological grades of breast cancer. Conclusion： ALDH1 and CD133 can be used as special markers of breast cancer stem cells.