上海市2005～2009年健康儿童肠道病毒携带状况调查

Enterovirus Carrying Status among Healthy Children under 5 Years Old in Shanghai from 2005-2009

目的了解上海市2005～2009年≤5岁健康儿童肠道病毒(Enterovirus,EV)携带状况。方法在EV感染的高发季节(6～8月),采集健康儿童粪便标本进行EV分离和血清型鉴定,对分离到的非脊髓灰质炎(Non-polio)EV(NPEV)测定VP_1编码区核苷酸序列以鉴定其血清型。结果从1630份粪便标本中共分离到18株脊灰病毒(Poliovirus,PV),阳性率为1.10%;分离到227株NPEV,阳性率为13.93%。PV经中国疾病预防控制中心病毒病预防控制所国家脊灰实验室型内鉴定均为疫苗株。上海市2005～2009年无脊灰野病毒的存在。上海市2005～2009年柯萨奇病毒B组3型(Coxsackievirus Group B Type3,CVB_3)形成二个分支,但都与欧美部分分离毒株有很大的差异。分离到的EV71型属于C_4基因亚型。结论上海市2005～2009年维持无脊灰状态。需逐步建立、完善上海市EV数据库,有助于了解EV在健康人群中的流行状况。

objective To explore the enterovirus （EV）carrying status among healthy children under 5 years old in Shanghai from 2005 to 2009. Methods during peak epidemic seasons for the EV （from July to Stool samples were collected from healthy children August）. EV was isolated from these stool samples and then was serotyped by neutralization using specific antisera. VP1 code gene of all non-polio EV （NPEV） isolated were sequenced in order to identify serotypes. Results 18 polioviruses were isolated from 1630 stool samples and carrying rate of poliovirus was 1.10%. 227 NPEV were isolated from the 1630 stool samples and carrying rate of NPEV was 13.93 % . Poliovirus were identified as vaccine strains by national laboratory of Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention. There was no wild type poliovirus in Shanghai from 2005 to 2009.Coxsackievirus group B type3 （CVB3）isolates from 2005 to 2009 in Shanghai formed two branch, but genetic diversity was found compared with CVB3 isolates from Europe and America. EV Type71 isolates were C4 subgenotype. Conclusion The EVcarrying rate was decline in healthy children in Shanghai from 2005 to 2009 and maintained polio-free status in Shanghai. We still need to establish and improve EV data bank in Shanghai to make futher realizing of the virus and its epidemic situation among healthy population.