COX-2介导槲皮素对UVB辐射引起的皮肤损伤的保护

Inhibition effect of quercetin on ultraviolet B radiation induced dermal damages through inhibiting the expression of COX-2

目的研究槲皮素对ultraviolet B(UVB)辐射引起的皮肤损伤的保护作用并探讨其可能的作用机制。方法分别建立小鼠和永生化的人角质形成细胞HaCaT的UVB损伤模型,研究体内和体外模型中槲皮素对受到紫外损伤的皮肤的保护作用。结果①经形态学观察,发现槲皮素能够显著缓解由UVB照射引起的皮肤水肿和炎症的发生。②动物组织切片免疫组织化学染色和细胞实时荧光定量PCR及Western blotting实验结果显示,UVB照射能够显著地提高皮肤组织和HaCaT细胞中COX-2的表达,而槲皮素能够在基因和蛋白水平显著地抑制UVB照射引起的COX-2的上调表达。结论槲皮素可能通过抑制诱导型环氧化酶COX-2的表达进而抑制UVB辐射引起的皮肤损伤。

Objective To investigate the protective effects of quercetin on ultraviolet B （UVB） radiation in- duced dermal damages and try to find the possible mechanisms. Methods The anti-inflammatory actions and the underlying mechanism of quercetin using cultured keratinocytes and mouse model were investigated. Re- sults ① By observing the morphology of the histological sections, it was found that UVB radiation signifi- cantly induced skin edema, and quercetin had a protective effect against this process just like the effect of sunscreen. ② The data of immunofluorescence staining, Real-time PCR and Western blotting showed that UVB radiation increased the expression of COX-2 significantly and quercetin could inhibit the process on gene and protein level. Conclusions Quercetin inhibits UVB radiation induced dermal damages through in- hibiting the expression of COX-2.