肉桂水提物对大鼠肠道梭菌属IV簇细菌和拟杆菌的影响

Effect of aqueous Cinnamomum cassia extracts on gastrointestinal Clostridium cluster IV and Bacteroides in rats

目的:肉桂水提物对大鼠结肠和直肠梭菌属IV簇和拟杆菌的影响。方法:给SD大鼠灌胃一个月后分别取结肠和直肠内容物,针对肠道梭菌属IV簇和拟杆菌的16SrRNA基因应用末端限制性片段多态性分析技术对这两类菌的多样性及分布进行分析。结果:研究发现肉桂水提物能降低梭菌属IV簇细菌在结肠和直肠中的数量,增加拟杆菌属细菌在结肠和直肠中的数量;该水提物对结肠和直肠的结构均产生影响,但影响并不相同,其中对直肠的影响更大。结论:肉桂水提物对大鼠肠道菌群的这种影响可能与肉桂治疗肥胖和糖尿病有功效有关。

Objective：To explore the impact of aqueous Cinnamomum cassia（C.cassia） extracts on gut microbiota.Methods：The colon and rectal contents were sampled after rats had been orally administrated the aqueous extracts for one month.Clostridium cluster IV and Bacteroides in the samples were analyzed by the terminal restriction fragment length polymorphism（tRFLP） method based on the bacterial 16S rRNA gene and nucleotide sequencing.Result：The aqueous C.cassia extracts could down-regulate the amount of microbiota of Clostridium cluster IV in rat gut,along with the increase of microbiota of Bacteroides.Various effects were exerted by the extracts on microbiota in colon and rectum,and the impact presented heavier in rectum.Conclusion：These variations of rat gut microbiota induced by the aqueous C.cassia extracts may reflect the functions of anti-obese and anti-dyspepsia of C.cassia.