五味子木脂素的提取优化及乳化稳定性研究

Research of Schisandra chinensis lignans extraction optimization and emulsion stability

以五味子种子为原料,提取溶剂为乙醇,采用超声波辅助提取工艺,并设计正交实验,优化得到了最佳的超声波提取工艺条件,然后以提取的五味子木脂素浸膏为原料,研究了吐温80、聚甘油辛癸酸酯、亲水性辛癸酸甘油酯对木脂素浸膏的乳化稳定性。研究结果表明,五味子木脂素的最佳提取工艺为:乙醇浓度90%,料液比(m:v)为1:8,提取时间为每次50min,提取次数为2次。在此条件下五味子木脂素的提取率为3.06%;木脂素乳化回填的最佳工艺为:每100mL乳液添加29.2mg木脂素浸膏,三种乳化剂用量分别为:吐温80,16.7mg;聚甘油辛癸酸酯,41.7mg;亲水性辛癸酸甘油酯,41.7mg。木脂素乳化回填后的溶液有较好的澄清度,并且在常温下存放60d未出现破乳现象,有较好的稳定性。

The application of ultrasonic extraction technology was studied with separated schisandra chinensis seed as raw material,ethanol as extraction solvent,and an orthogonal experiment scheme was designed to optimize to the best extraction process.Then the effect of twain 80,polyglycerol essien acid ester compounds,hydrophilic octyl and decyl glycerate to emulsion stability was studied with lignans extracts as raw material.The result of the study showed that,the best extraction process of Schisandra chinensis：concentration of ethanol：90%,solid-liquid ratio：1：8,extraction time：50min,extraction frequency：twice.Under these conditions,the extraction rate of Schisandra lignans was 3.06%.The best technology of add-back process of emulsification：29.2mg lignans extract was added to every 100mL emulsion and twain 16.7mg,Polyglycerol essien acid ester compounds,41.7mg and hydrophilic octyl and decyl glycerate,41.7mg were also needed.Solution after lignans emulsification backfilling was clear,and had better stability since the emulsification phenomenon in 60 days stored at room temperature didn＇t appear.