肝硬化患者外周血IL-8及其mRNA表达与HBV载量相关性

Expression of IL-8 and its mRNA in peripheral blood of patients with cirrhosis and their correlation with hepatitis B viral load

目的探讨肝硬化患者血清IL-8含量、外周血单个核细胞(PBMCs)中IL-8mRNA表达及其与HBV载量相关性。方法以ELISA法检测65例肝硬化患者(代偿期36例,失代偿期29例)血清IL-8含量,定量PCR检测患者外周血IL-8mRNA水平,设GAPDH为参照,以logcDNA/logGAPDH比值代表其最终mRNA水平。以Real-time PCR法检测患者外周血HBV载量。结果肝硬化患者外周血IL-8及其mRNA表达量分别为(448.41±59.46)pg/mL、(1.485 0±0.180 1)logcD-NA/logGAPDH,较正常组显著增高,差异有显著性(P<0.01)。其中,失代偿组IL-8及其mRNA分别为(488.19±49.44)pg/mL、(1.545 3±0.178 3)logcDNA/logGAPDH,较代偿组升高,差异有显著性(P<0.01,P<0.05)。病毒复制组外周血IL-8及其mRNA水平分别为(472.21±52.44)pg/mL、(1.567 6±0.155 8)logcDNA/logGAPDH,与HBV载量相关系数分别为0.487、0.454,较非复制组显著升高,差异有统计学意义(P<0.01)。结论肝硬化外周血IL-8及其mRNA表达升高,不仅与体内病毒复制水平密切相关,且与患者病情严重程度有关。IL-8在肝硬化的致炎细胞分子病理损伤过程中起重要作用。

The purpose of this paper is to study the expression of IL-8 in serum and IL-8 mRNA in peripheral blood mononuclear cells（PBMCs）,and the correlation with hepatitis B viral load in the patients with cirrhosis.The levels of IL-8 in serum of 65 patients with cirrhosis（36 with compensated cirrhosis,29 with decompensated cirrhosis） were detected by ELISA,and the expression of IL-8 mRNA in the peripheral blood was simultaneously explored by the quantitative PCR.The ratio of logcDNA/logGAPDH was regarded as the extreme level of IL-8 mRNA.The hepatitis B viral load in PBMCs and serum were detected by Real-time PCR.The results showed that the levels of IL-8 and its mRNA in peripheral blood of the patients were（448.41±59.46） pg/mL,（1.4850±0.1801） logcDNA/logGAPDH,which were both significantly higher than those in normal controls（P0.01）.The high levels of IL-8（488.19±49.44）pg/mL and its mRNA（1.5 453±0.1783） logcDNA/logGAPDH both were identified higher in decompensated cirrhosis than those in the compensated cirrhosis（P0.01）.They were also significantly higher in the group with positive of HBV-DNA（472.21±52.41 pg/mL,1.5 676±0.1 558 logcDNA/logGAPDH） than those in the group with negative of HBV-DNA（P0.01）.The correlation coefficients between CXCR1,CXCR2 and the loads of HBV were 0.487 and 0.454,respectively.It is concluded that the high levels of IL-8 and their mRNA expression are confirmed in patients with cirrhosis and have significantly associated with the hepatitis B viral load.Moreover,they also related with the disease progression of cirrhosis.The IL-8,a key type of inflammatory cytokine or chemokine,may play an important role in the cellular and molecular mechanisms of pathological damage in cirrhosis.