兔VX2肝癌模型制作技术改良

Comparison of three induction methods of VX2 hepatoma in rabbits

目的探讨制作兔VX2肝癌模型的新技术、新方法。方法将新西兰兔30只随机等分为3组,分别为嵌插组、改良嵌插组和经皮穿剌组。分别按不同方法建立肝癌模型,于建模后第1、2、3周分别进行CT扫描,评估肿瘤大小。3周后处死动物进行解剖,观察肝脏成瘤及转移情况,比较各组转移率,并行常规病理组织学检查。结果 30只动物建模成功28只,成功率93%,经皮穿剌组有2只肝脏未见种植灶,嵌插组和改良嵌插组全部种植成功。改良嵌插组腹壁转移率显著低于嵌插组和经皮接种组。结论改良嵌插法3周内不会形成接种部位腹壁以及远处的转移,是一种理想的移植性肝癌模型建模方法。

Objective To compare three induction methods of VX2 carcinoma in rabbits.Methods Thirty New Zealand rabbits were randomly divided into 3 groups： impaction embedding group,modified impaction embedding group and percutaneous puncture implantation group.The VX2 models were established separately and CT evaluation was carried out at 1,2,and 3 weeks after tumor implantation.All the animals were sacrificed and tissues were harvested 3 weeks after implantation to compare the success rate and the metastasis rate in the abdominal wall and other organs.Results The success rate was 93%（28/30） and both the two failed cases were in the percutaneous puncture implantation group.Relative to the other groups,the abdominal wall metastasis rate of the modified impaction embedding group is obviously lower.Conclusions The modified impaction embedding technique is a promising novel technique in preparation of VX2 hepatoma in rabbits with a high success rate and low abdominal wall metastasis rate.