应用MRI预扫描定位提高大鼠脑立体定位术的准确性

Value of MRI Pre-Scanning in Improving the Accuracy of Stereotactic Brain Puncture in Rats

目的评价MRI预扫描方法在鼠脑穿刺定位准确性上的作用与价值。材料与方法选取健康雄性SD大鼠18只,随机分为2组,分别为MRI预扫描定位组(9只),在MR图像指导下进行穿刺注射;以及经典穿刺组(9只),不采用MR影像学检测定位,严格按照脑立体定位图谱进行穿刺注射。穿刺以通过苍白球中心的鼠脑切面中尾状核区为靶目标,穿刺后采用MRI影像学方法确定穿刺点位置,比较MRI预扫描定位穿刺与经典立体定位穿刺的准确度。结果 MRI预扫描定位组穿刺准确率为88.89%,经典穿刺组穿刺准确率为66.67%;两种方法的穿刺定位差异有显著性(P<0.05),MRI预扫描组的穿刺定位准确度明显高于经典穿刺组。结论 MRI预扫描可显著提高鼠脑立体定位穿刺的准确度,是进行立体定位精细穿刺的必要操作。

Objective To evaluate the value of MRI pre-scanning technique in stereotactic brain puncture in rats.Methods Totally 18 SD rats were randomly divided into 2 groups（n=9）：control group,in which the brain puncture was performed in strict accordance with the stereotactic brain atlas,and experimental group,in which MRI pre-scanning was performed and brain puncture was guided by the images.Rats in both groups were injected 2μL Gd-DTPA,taking caudate nucleus in the axial section which crosses the center of globus pallidus as target.Then MRI scanning was performed with three-dimensional magnetization-prepared rapid gradient-echo sequence to detect the enhancement of Gd-DTPA and compare the brain puncture accuracy between the two groups.Results The number of rats which were located precisely was 8 in experimental group（88.89%）,and 3 in the control group（66.67%）.The difference between the two groups was statistically significant（P〈0.05）.The accuracy of experimental group was much higher than that of control group.Conclusion MRI pre-scanning can improve the accuracy of stereotactic brain puncture,and it is a necessary step to confirm accuracy and repeatability of the stereotactic operations.