桑枝对小鼠脑缺血再灌注损伤的保护作用

Protection Effect of Mulberry on Cerebral Ischemia-Reperfusion Injury in Mice Brain Tissue

目的:观察桑枝对小鼠脑缺血再灌注损伤的保护作用。方法:将小鼠分为4组,假手术组;模型组(分离两侧颈总动脉及迷走神经,用微型动脉夹同时夹闭双侧颈总动脉,制作小鼠脑缺血再灌注模型);阳性药组给以尼莫地平24mg/kg,受试药组给以桑枝水提醇沉液14g生药/kg,均灌胃给药7天,24h后取材,匀浆,测脑组织Evan's蓝含量及抗氧化酶SOD活性与MDA水平的变化。结果:缺血再灌注后,脑组织Evan's蓝含量升高,给药后降低。脑缺血再灌注组SOD活性降低,MDA含量增高,而治疗组SOD活性增高,MDA水平降低。结论:桑枝能增加脑缺血再灌注小鼠SOD的活性,改善脑血流,减轻血脑屏障的损伤,对脑缺血再灌注损伤具保护作用。

Objective ：To investigate the protection of Mulberry on cerebral ischemia -reperfusion injury in mice brain tissue. Methods ：The mice were divided into four groups：control group; model group（ separate carotid artery and the vagus nerve,use micro- artery occlusion folder on bilateral carotid artery, reproduce mice brain ischemia -reperfusion model）, positive drug group （nimodipine 20mg/kg） ; tested drug Mulberry group （20mg/kg）. Every group took intragastric adminis- tration,once a day for seven days. Brain tissue Evan~ Blue content and antioxidant enzymes and other changes were detec- ted. Result：Evan~ Blue content in the brain tissue increased in model group, meanwhile, it decreased after nimodipine and Mulberry administration. SOD activity of cerebral ischemia - reperfusion group decreased and MDA levels increased, but in the treatment group the activity of SOD increased, and MDA decreased. Conclusion：Mulberry can increase the activity of SOD,improve cerebral blood flow and reduce the blood - brain barrier damage in cerebral ischemia - reperfusion of mice.