克痛药膜中元胡大黄姜黄血竭的薄层色谱研究

Study on Corydalis Yanhusuo Rhubarb Curcuma and Dragon's Blood in Pain-Restraining Medicinal Membrane by Thin-layer Chromatography

目的:研究克痛药膜中的元胡、血竭、姜黄、大黄4味中药的薄层色谱。方法:采用薄层色谱法,在硅胶G薄层板上,以环己烷-丙酮(8∶2)为展开剂,鉴别元胡;以石油醚(60～90℃)-乙醚(3∶2)为展开剂,鉴别大黄;以三氯甲烷-甲醇-甲酸(96∶4∶0.7)为展开剂为展开剂,鉴别姜黄;以三氯甲烷-甲醇(19∶1)为展开剂,鉴别血竭。结果:依据正文所述方法,薄层展开后,色谱斑点显色清晰,分离效果好,与对照药材显色一致。结论:依据正文所述方法,针对元胡、血竭、姜黄、大黄4味中药,其薄层色谱图的检出成分消除了其它成分的干扰,专属性强,重现性好,方法简便,可以作为该制剂质量控制的检测标准。

Objective： To research corydalis yanhusuo, rhubarb, curcuma and dragon~ blood in pain -restraining medici- nal membrane by thin - layer chromatography. Methods ： Adopting the thin - layer chromatography, on the thin layer of the silicon G,identified corydalis yanhusuo using eyclohexane- acetone（8：2） as the developing agent;identified rhubarb using petroleum ether（ 60 ~ 90~C ） - aether（ 3 ： 2） as the developing agent; identified curcuma using chloroform - methanol - meth- anoic acid（96：4：0.7） as the developing agent;identified dragon~ blood using chloroform- methanol （19：1 ）as the develo- ping agent. Results ：According to the related method, after the thin layer expanded, every main fleck was clearly colored, well disparted and have the same color with the respective contrast medicinal materials~ main flecks. Conclusions ： Accord- ing to the related method, the thin -layer chromatography clearly showed the component of corydalis yanhusuo, rhubarb, cur- cuma and dragon~ blood which were distinctly detected without the interference of other elements. The related method was specific ,repeatable, simple ,and could be taken as the standard to control the quality of this preparation.