阴道白假丝酵母菌酶活性影响因素的研究

The influence factor on the enzyme activity in Candida albicans

目的:探讨外环境改变对阴道白假丝酵母菌酵母相和菌丝相的酶活性的影响。方法:收集标本50株菌株,用牛奶培养基和卵黄培养基法检测白假丝酵母菌酵母相与菌丝相分泌型酸性蛋白酶(SAP)和磷脂酶(PL)的酶活性;从中随机挑选20株菌株,分别用不同pH值的培养基检测其酵母相和菌丝相酶活性的改变;在不同乳杆菌浓度的环境下检测阴道白假丝酵母菌酵母相和菌丝相酶活性的变化。结果:酵母相和菌丝相磷脂酶的活性(PZ值)分别为0.546±0.132和0.471±0.119;分泌型酸性蛋白酶的活性(PA值)分别为0.605±0.125和0.568±0.086;菌丝相分泌型酸性蛋白酶和磷脂酶活性均显著高于酵母相(P<0.001)。pH=4时,白假丝酵母菌酶活性最低,随着pH值增加,酶活性升高,至pH=7时酶活性降低。未添加乳杆菌组白假丝酵母菌酶活性最高,逐渐增加乳杆菌浓度,酶活性随之降低。结论:菌丝相酶活性均高于酵母相;pH值可影响白假丝酵母菌酶活性的改变,过酸或过碱的环境均可抑制白假丝酵母菌酶活性;乳杆菌对白假丝酵母菌的酶活性有抑制作用,且对菌丝相的抑制作用较酵母相更强。

Objective：To investigate the influence of external environment alteration on the enzymic activity of dimorphism in Candida albicans.Method：50 strains of Candida albicans were collected.Milk-plate medium and egg-yolk medium were used respectively to test the activities of secretory acid proteinase and extracellular phospholipase of Candida albicans in hyphal form and in spore form.20 strains of Candida albicans were used to test the enzymic activity cultured with different pH value.The enzymic activity was detected in hyphal form and in spore form after addition of in different concentrations of the Lactobacillus.Results：The value of PZ was 0.546±0.132 in spore form and 0.471±0.119 in hyphal form.The value of PA was 0.605±0.125 in spore form and 0.568±0.086 in hyphal form.The activities of secretory acid proteinase and extracellular phospolipase between the two forms were significantly different（P0.001）.The enzymic activity of Candida albicans was lowest when pH=4,the enzyme activity increased with the pH value increased,to pH=7 the enzyme activity reduced.The enzymic activity of Candida albicans was highest in the no Lactobacillus group and the enzyme activity would decrease as Lactobacillus concentration was added gradually.Conclusion：The activities of secretory acid proteinase and phospolipase in hyphal form are significantly higher than those in spore form.The enzyme activity can be influenced by pH value and it is restrained in overly acidic or alkaline environments.The enzyme activity is inhibited by Lactobacillus and the inhibition in hyphae is stronger than in yeast.