酶解大豆蛋白产天冬氨酸蛋白酶抑制剂的研究

The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases

本文通过采用可控酶解的方式酶解大豆蛋白获得了一种源自大豆的天冬氨酸蛋白酶抑制剂SAPI(soyaspartic proteinase inhibitor)。酶解液经过DEAE-52阴离子交换层析、Superdex Peptide 10/300 GL凝胶层析、SOURCE 15RPC反相层析分离后最终比抑制活力为254.2 IU/mg,纯化倍数为62。SAPI对胃蛋白酶(3000U)半数抑制浓度IC50为25.67μg/mL,有良好的热稳定性,属于一种非竞争性抑制剂。

In this paper, we reported a kind of aspartic protease inhibitor from soy protein （ soy aspartie proteinase inhibi- tor,SAPI） after controlled enzymatic hydrolysis. The final inhibitory activity was 2-4.2 IU/mg and the purification fold was 62 after DEAE-52 ion-exchange chromatography, Superdex Peptide 10/300 GL gel chromatography and SOURCE 15RPC reversed-phase chromatography. The IC50 value of SAPI was 25.67 μg/mL to pepsin（3000 U） ,SAPI showed good thermostability and belonged to a kind of non-competitive inhibitor.