凹叶厚朴抑制α-糖苷酶与抗氧化活性研究

α-Glucosidase Inhibitory and Antioxidant Activity of Magnolia Officinalis

首次采用96微孔板法检测贵州和河南产凹叶厚朴抑制α-葡萄糖苷酶活性;并采用DPPH、ABTS和FRAP三种方法测定其抗氧化活性。贵州产凹叶厚朴乙酸乙酯(IC50=7.22μg/mL)和正丁醇提取部位(IC50=36.59μg/mL),河南产凹叶厚朴石油醚(IC50=107.04μg/mL)和乙酸乙酯提取部位(IC50=17.17μg/mL),它们的活性都远高于于阳性对照Acarbose(IC50=1081.27μg/mL)。贵州产凹叶厚朴乙酸乙酯提取部位清除ABTS自由基的能力最强(IC50=8.81μg/mL),强于阳性对照BHT(IC50=11.94μg/mL);其次为河南产凹叶厚朴乙酸乙酯提取部位(IC50=12.73μg/mL)。研究结果表明,贵州产凹叶厚朴乙酸乙酯提取部位抑制α-葡萄糖苷酶和抗氧化活性最好。

The α-glucosidase inhibitory activities of Magnolia Officinalis from Guizhou and Henan province was assayed by the method of 96-microplates for the first time, and its antioxidant activity was determined by the method of DPPH, ABTS and FRAP. Ethyl acetate （ IC50 = 7.22 μg/mL） and methanolic extracts （ IC50 = 36.59 μg/mL） of M. offwinalis from Guizhou,and the petroleum ether （ICs0 = 107.04 μg/mL） and ethyl acetate extracts （IC50 = 17.17 μg/mL） of M. officinalis from Henan were higher than that of Acarbose （IC50 = 1081.27 μg/mL） as positive control. Ethyl acetate extracts of M. offwinalis from Guizhou had the highest ABTS free radical scavenging activity （ICso = 8.81 μg/mL）, which was higher than that of BHT（IC50 = 11.94 μg/mL）as positive control, and followed by ethyl acetate extracts of Magnolia Officinalis from Henan （ ICso = 12.73 μg/mL）. The results indicated that ethyl acetate extracts of M. officina- I/s from Guizhou showed the strongest activity of a-glucosidase inhibitory activity and antioxidant activity.