绵羊催乳素受体基因外显子10多态性及其与产羔数相关分析

Analysis on Genetic Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Litter Size of Sheep

采用PCR-SSCP技术检测催乳素受体(PRLR)基因外显子10在湖羊、小尾寒羊、洼地羊和阿勒泰羊中的单核苷酸多态性,研究该基因与湖羊高繁殖力的关联性。结果表明:4个绵羊品种在P2引物扩增区域均符合Hardy-Wenberg平衡,但P3引物只有阿勒泰羊符合Hardy-Wenberg平衡。P2扩增片段的不同基因型分布频率在4个品种间差异不显著(P>0.05);而除阿勒泰羊P3扩增片段的基因型分布与其余3个品种间存在极显著差异(P<0.01)外,其余各品种间基因型分布差异不显著(P>0.05)。4个绵羊品种间产羔数存在极显著差异(P<0.01),但不同基因型间产羔数差异不显著(P>0.05);湖羊不同引物不同基因型产羔数的差异亦均不显著(P>0.05)。本研究表明,PRLR基因不能作为湖羊高繁殖力的候选基因。

Prolactin receptor （PRLR）gene was studied as a candidate gene for the prolificacy of Hu sheep. Primers were designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene in Hu sheep, Small Tail Han sheep, Wadi sheep and Altay sheep by PCR-SSCP. The products amplified by P2 of the four sheep and P3 of Altay sheep were accorded with Hardy -Wenberg equilibrium, and others were in Hardy-Wenberg disequilibrium. In the fragment amplified by primer P2 there was no significant difference for the distribution frequency among different genotypes of the four sheep breeds （P〉 0.05）. In the fragment amplified by primer P3 there was extremely significant difference for the distribution frequency between Altay sheep and the other three breeds （P〈0.01）, and there were no significant differences among other three breeds （P〉 0.05）. There were extremely significant difference for the litter size among four breeds（P〈0.01）, but no significant difference was observed among different genotypes amplified by primer P2 and primer P3 （P〉0.05）. There was no significant difference among the different genotypes for the litter sizes of Hu sheep （P〉0.05）. The research showed that PRLR gene couldn＇t be selected as a candidate gene for the prolificacy of Hu sheep.