脑红蛋白在小儿脑挫裂伤病理过程中变化的初步研究

Changes of neuroglobin in the pathologic process of contusion and laceration of brain in children

目的初步探讨脑红蛋白(neuroglobin,Ngb)在小儿脑挫裂伤病理生理过程中的作用。方法采用二维凝胶电泳技术分离3例行脑室肿瘤切除患儿的正常额叶皮层组织和8例脑挫裂伤患儿额叶皮层组织总蛋白质,PDquest图像分析软件识别差异蛋白质点,电喷雾串联质谱鉴定差异蛋白;免疫组化检测差异蛋白Ngb在正常脑组织和脑挫裂伤患儿的脑组织中的表达。并采用酶联免疫法检测10例正常儿童和15例脑挫裂伤患儿血清中Ngb的表达变化。结果建立了正常儿童和脑挫裂伤患儿脑组织的二维凝胶电泳图谱,识别了6个差异蛋白质点,并鉴定了5种差异蛋白。免疫组化结果显示,Ngb在脑挫裂伤患儿额叶皮层组织中的表达水平高于正常对照组额叶皮层组织(P<0.05)。酶联免疫法检测结果表明,脑挫裂伤后6 h、12 h、18 h、24 h及48 h血清Ngb表达高于正常对照组,差异有统计学意义(P<0.01)。结论 Ngb可能在小儿脑挫裂伤病理生理过程中发挥了重要作用。

Objective To study the role of neuroglobin（Ngb） in the pathologic process of contusion and laceration of brain in children.Methods The proteins in the brain tissue were extracted by two-dimensional gel electrophoresis in 3 children undergoing brain ventricular neoplasms resection（normal brain tissue） and in 8 children with contusion and laceration of brain.The image analysis was done using the PDQuest 7.0 software.The differential protein spots were detected and analyzed with Applied Biosystems Voyager System 4307 MALDI-TOF Mass Spectrometer and bioinformatical skills.Ngb expression in the brain tissue was measured using immunohistochemisty.Ngb expression in plasma was measured using ELISA in 15 children with contusion and laceration of brain and 10 healthy children.Results Expression maps of the brain tissue were established by two-dimensional gel electrophoresis in children with contusion and laceration of brain and healthy children.Six differential protein spots were found and 5 of them were identified by mass spectrum.Immunohistochemisty assay showed that Ngb expression in the brain tissue in children with contusion and laceration of brain was significantly higher than in normal controls（P0.05）.ELISA results showed that Ngb expression in the plasma increased significantly 6,12,18,24 and 48 hours after trauma in children with contusion and laceration of brain compared with healthy children（P0.01）.Conclusions Ngb may play an important role in the pathologic process of contusion and laceration of brain in children.