基于表面等离子共振GGBP胺耦合绑定的葡萄糖浓度测量影响因素

Influencing Factors in Glucose Concentration Detection by Surface Plasmon Resonance with GGBP Amine Coupling

人体组织液中的葡萄糖浓度与血糖浓度密切相关,微创血糖检测通过测量组织液中的葡萄糖浓度来预测血糖浓度,而组织液中成分复杂,利用对葡萄糖有特异性吸附作用的D-半乳糖/D-葡萄糖结合蛋白(GGBP)胺耦合绑定修饰表面等离子共振(SPR)传感器可以实现葡萄糖选择性测量.但是由于测量过程复杂,需要对影响测量精度的因素进行评价,确定最佳测量条件,以微型SPR传感器为核心搭建了一套葡萄糖浓度测量系统,系统地分析研究了GGBP胺耦合绑定SPR葡萄糖浓度测量过程中的各种影响因素,在不同实验条件下进行了测量实验,获得了蛋白质纯化、蛋白质活性、基线、流速、传质效应、温度和气泡等对测量结果的影响机制,提出了相应的解决方案,优化了测量流程,为标准化和小型化仪器开发提供重要依据.

The glucose concentration of human interstitial fluid is closely related to that of human blood. The minimally invasive blood glucose monitoring can be realized by measuring the glucose con- centration of interstitial fluid. Considering the complexity of the interstitial fluid, the D-galactose/D- glucose binding protein （GGBP） which can specifically absorb glucose molecules can be used to modify the surface plasmon resonance （SPR） sensor with amine coupling method to realize glucose selective measurement. The measuring process is complicated, so the factors which influence the measurement accuracy need to be evaluated to obtain the optimized measurement condition. A SPR glucose concen- tration detection system based on a miniature integrated SPR sensor was set up, the factors affecting the measurement results were studied systemically by experiments, including protein purification, pro- tein activity, baseline, flow velocity, mass transfer effect, temperature and bubble, and some solutions were proposed accordingly. The study optimizes the measurement process and provides important in- struction for the standardization and development of miniaturization instrument.