血清基质金属蛋白酶-9在乳腺癌中的表达及临床意义

Expression and Clinical Significance of Serum Matrix Metalloproteinases-9(MMP-9) in Breast Cancer

目的探讨乳腺癌患者血清基质金属蛋白酶-9(MMP-9)抗体表达水平及其临床意义。方法采用酶联免疫吸附法(ELISA)分别检测70例乳腺癌、35例乳腺良性病变、30例健康体检者血清MMP-9抗体浓度,同时采用免疫组化EnVision分别检测各组标本中MMP-9的表达水平,以了解两种检测方法的一致性,分析血清MMP-9抗体与乳腺癌临床病理参数的关系。结果 ELISA检测血清MMP-9抗体在乳腺癌标本中的表达均明显高于乳腺良性病变和健康体检者,差异有统计学意义(P<0.01);EnVision检测血清MMP-9在健康体检组、乳腺良性病变组、乳腺癌组中的表达分别为阴性、弱阳性和强阳性,这两种检测方法结果呈一致性(P<0.01)。血清MMP-9的表达与乳腺癌的年龄、肿瘤直径、组织学类型、组织学分级及人表皮生长因子受体-2(HER-2)无关(P>0.05),而与淋巴结转移、雌激素受体(ER)及孕激素受体(PR)表达有关(P<0.01)。血清MMP-9的表达与ER、PR呈正相关(P<0.01)。结论乳腺癌患者血清MMP-9表达水平明显升高,因此,检测血清MMP-9对乳腺癌的预后及病情评估有重要的价值。

Objective To explore the expressions and clinical significance of MMP-9 antibody in blood serum in treating patients with breast cancer.Methods Eenzyme-linked immunosorbent assay（ELISA） was taken to detect MMP-9 antibody concentration in breast cancer（70 cases）,benign breast diseases（35 cases） and healthy women（30 cases）.At the same time MMP-9 was tested by EnVision for every group to study the consistency between ELISA and EnVision,and to analyze the relationships between MMP-9 antibody and the clinical pathological factors of breast cancer.Results The expression of serum MMP-9 antibody tested by ELISA in breast cancer was significantly higher than that in benign breast diseases and healthy women（P0.01）;The expressions of MMP-9 tested by EnVision in healthy women group,benign breast diseases group and breast cancer group were negative,weakly positive and strongly positive respectively;The result of the two defferent test methods was consistent（P0.01）.The expression of serum MMP-9 antibody had no statistical difference in age,tumor size,histological category,histological grade and HER-2（P0.05）,but was related to lymphatic metastasize,the expression of estrogen receptor（ER） and progesterone receptor（PR）（P0.01）.There was direct correlation between the expression of serum MMP-9 and ER,PR（P0.01）.Conclusion The blood serum levels of MMP-9 antibody in breast cancer increase remarkably,so the detection of serum levels of MMP-9 is of great value in evaluations of prognosis and pathogenetic condition.