黑龙江省长白落叶松八齿小蠹虫体及坑道的真菌类群

Fungal population in the Ips subelongatus body and gallery of Larix olgensis in Heilongjiang Province

采集落叶松八齿小蠹成虫、幼虫、蛹,以及母坑道、子坑道、蛹室的韧皮组织和蓝变组织。采用马丁氏培养基和PDA综合培养基对各虫态体表、体内、坑道内真菌进行分离培养,统计真菌检出率。对获得的真菌菌株通过形态学和分子生物学相结合的方法进行鉴定。在落叶松八齿小蠹虫体内外和坑道内共分离出真菌24种,其中子囊菌7种,担子菌2种,无性型真菌15种。从各虫态体表共分离出真菌21种,从体内共分离出真菌6种,24种真菌在坑道内均有检出。其中,Ceratocystis fujiensis为检出率较高的蓝变真菌。

Samples of adult, larvae and pupa of Ips subelongatus, and the blue-stained tissue and phloem of Larix olgensis bearing pupa cells, egg and larval galleries were collected. The fungi in the inside and outside of the insect body and gallery of larch log were cultured and isolated by Martin and PDA media, and the detection frequency of the fungi were counted. A total of 24 fungal species were isolated and identified, of which 7 species belong to Ascomycota, 2 species Basidiomycota and 15 species anamorphic fungi. All these fungi can be isolated from the insect-bored gallery, 21 species from the body surface of the beetle, and 6 species from the inside of the body. Ceratocystis fujiensis was the most frequently isolated fungus.