单核苷酸多态性与胃癌易感性的相关性研究

Correlation between single nucleotide polymorphisms and susceptibility of gastric cancer

目的研究单核苷酸多态性与胃癌易感性的相关性。方法选取2009年5月～2012年5月笔者所在医院收治的242例胃癌患者血样(实验组)与344例来自健康人群的正常血样(对照组)。对两组血样EZH2rs734004、rs734005、rs2072407、rs6464926、rs12670401多态性测序,运用组织芯片技术与免疫组化检测240例同一来源胃癌组织与30例正常胃黏膜EZH2蛋白表达。结果检测发现EZH2中rs12670401基因型CC与rs6464926基因型TT在胃癌患者中所占的比例较高,EZH2与rs2072404比例增加会严重影响到胃癌发生率与风险(P=0.007,OR=1.785,95%CI=1.171～2.724;P=0.012,OR=1.720,95%CI=1.121～2.623)。胃癌患者中的EZH2包含的杂合基因型rs2072407TC、rs734004TC、rs734004CG所占比例低于正常血液对照组,差异有统计学意义(P<0.05)。所占比例减少可降低患者胃癌发生的几率与风险(P=0.032,OR=0.712,95%CI=0.520～0.975;P=0.033,OR=0.708,95%CI=0.517～0.971;P=0.035,OR=0.712,95%CI=0.517～0.977)。应用连锁不平衡分析LD进一步对上述观察进行证实。胃癌不同组织学类型与不同临床参数间的EZH2免疫组化染色强度差异无统计学意义(P>0.05)。结论单核甘酸多态性与胃癌易感性相关。

Objective To study correlation between single nucleotide polymorphisms and susceptibility of gastric cancer. Methods 242 patients with gastric cancer samples and 344 cases of normal blood group of our hospital from May 2009 to May 2012 were selected. EZH2 rs734004, rs734005, rs2072407, rs6464926, rs12670401 of two groups were polymorphism DNA sequenced. EZH2 protein expression of 240 cases of the same source of gastric carcinoma and 30 cases of normal gastric were detected using tissue microarray technique and immunohistochemical technique. Results The rs12670401genotype CC and rs6464926 genotype TT of EZH2 in patients with gastric cancer in proportion were higher. The increase of EZH2 and rs2072404 in proportion can affect the probability and risk of gastric cancer（P=0.007,OR=1.785,95% CI=1.171-2.724. P=0.012,OR=1.720,95% CI=1.121-2.623）. In the patients with gastric cancer, EZH2 contained heterozygous genotypes rs2072407TC, rs734004TC, rs734004CG,which was lower than that of the normal blood group. The difference has statistical significance. The proportion of patients with gastric reduction may reduce the probability and risk （P=0.032, OR=0.712, 95%CI=0.520-0.975.P=0.033,0R=0.708,95%CI=0.517-0.971.P=0.035,OR=0.712,95%CI=0.517-0.977）.LD of linkage disequilibrium analysis was used to prove the further official. Immunohistochemical staining intensity of EZH2 of gastric cancer of different histological types and different clinical parameters had no significant difference. Conclusion Single nucleotide polymorphisms and susceptibility of gastric cancer were related.