“百会”透“曲鬓”头针疗法对急性脑出血大鼠脑组织GDNF及VEGF表达的影响

Effects of the Scalp Acupuncture at Baihui(DU20) through Qubin(GB7) on the Expressions of GDNF VEGF in the Brain Tissue of Rats with Acute Intracerebral Hemorrhage

目的观察"百会"透"曲鬓"头针疗法对急性脑出血大鼠脑组织胶质源性神经营养因子(GDNF)和血管内皮生长因子(VEGF)表达的影响。方法选取健康雄性Wistar大鼠150只制备脑出血模型,随机分为模型组、针刺组、西药组,每组50只;每组再随机分为6h、1d、2d、3d、7d五个亚组,每个亚组10只;另设10只正常大鼠作为空白组。针刺组捆绑固定,针刺患侧"百会"透"曲鬓"穴。西药组给予茴拉西坦稀释液1mL灌胃,3次/d。模型组大鼠给予针刺组相同捆绑30min/d,生理盐水1mL灌胃,3次/d。运用原位杂交及免疫组化法检测各组大鼠脑组织中GDNF和VEGF阳性细胞的表达。结果与空白组比较,模型组6h～3dGDNF阳性细胞数增多,各时间点VEGF阳性细胞数增多,差异均有统计学意义(P<0.01)。针刺组各时间点GDNF阳性细胞数均较模型组和西药组增多,差异均有统计学意义(P<0.01);西药组各时间点与模型组比较,差异均无统计学意义(P>0.05)。与模型组比较,针刺组6h～3dVEGF阳性细胞数减少,7d时间点VEGF阳性细胞数增多,且高于西药组同期,差异均有统计学意义(P<0.01)。结论 "百会"透"曲鬓"头针疗法可能在脑出血急性期通过促进内源性GDNF表达、早期抑制VEGF的表达并后期促进VEGF的表达等途径发挥神经重塑作用。该方法可能在脑出血急性期具有良性的双向调节作用,疗效优于茴拉西坦。

Objective To observe the effects of the scalp acupuncture at Baihui （DU20~） through Qubin GB7） on the expressions of glial cell line-derived neutrotrophic factor （GDNF） and vascular endothelial growth factor （VEGF） in the brain tissue of rats with acute intracerebral hemorrhage （AICH）. Methods Totally 150 healthy male Wistar rats were used to prepare the intracerebral hemorrhage （ICH） model. They were randomly divided into the model group, the acupuncture group, and the Western medicine group, 50 in each group. Rats in each group were then randomly divided into five subgroups, i. e., 6 h, day 1, day 2, day 3, and day 7, 10 in each subgroup. Another 10 normal rats were recruited as the blank control group. Rats in the acupuncture group were acupunctured at Baihui （DU20） through Qubin （GB7） on the lesion side. Rats in the Western medicine group were administered with aniracetam diluent 1 mL by gastrogavage, 3 times daily. Rats in the model group were bound the same way as those in the acupuncture group 30 min daily, and then administered with normal sa- line 1 mL by gastrogavage, 3 times daily. The expressions of GDNF and VEGF in the brain tissue of the rats in each group were detected using in situ hybridization and immunohistochemical methods. Results Compared with the blank control group, the number of GDNF positive cells in the model group increased at 6 h-3 days, and the number of VEGF positive cells in the model group increased at each time point, showing statistical difference （ P〈0.01 ）. The numbers of GDNF positive cells increased in the acupuncture group at each time point, showing statistical difference when compared with those of the model group and the Western medicine group （P 〈0.01 ）. There was no statistical difference between the model group and the Western medicine group at each time point （P〉0.05）. Compared with the model group, the number of VEGF positive cells in the acupuncture group de- creased at 6 h -3 days. The VEGF positive cells increased at day 7 and were higher than that of the Western medicine group, showing statistical difference （P 〈 0.01 ）. Conclusions The scalp acupuncture at Baihui （DU20） through Qubin （GB7） played the role of nerves remodeling in AICH possibly through promoting the ex- pression of endogenous GDNF and early inhibiting the expression of VEGF in the AICH. This method possibly had favorable bidirectional regulation effects in AICH. Its therapeutic effects were superior to Aniracetam.