经前平颗粒对经前期综合征肝气逆证大鼠脑μ阿片受体mRNA及蛋白表达的影响

Effects of Jingqianping Granule on mRNA and Protein Expression of μ Opioid Receptor in Premenstrual Syndrome Gan-qi Invasion Rats

目的观察经前平颗粒对经前期综合征(premenstrual syndrome,PMS)肝气逆证大鼠脑顶区皮质、额区皮质、下丘脑、海马μ阿片受体(μ opioid receptor,MOR)mRNA及蛋白的表达的影响。方法取20只大鼠制备PMS肝气逆证大鼠模型。造模后大鼠分为模型组、中药组,每组10只。另选取10只大鼠作为正常对照组(正常组)。造模同时,中药组大鼠给予经前平颗粒(1.6g/kg)灌胃,正常组和模型组大鼠给予等体积生理盐水(1mL/100g)灌胃,每天1次,连续5天。采用RT-PCR和Western blot法分别检测大鼠顶区皮质、额区皮质、下丘脑和海马MOR mRNA及蛋白表达。结果与正常组比较,模型组大鼠顶区皮质、额区皮质MOR mRNA及蛋白扩增产物电泳条带相对较弱,光密度值降低,顶区皮质、额区皮质MOR mRNA及蛋白相对表达降低;下丘脑、海马MOR mRNA及蛋白电泳条带相对较强,光密度值升高;下丘脑、海马MORmR-NA及蛋白相对表达升高,差异均有统计学意义(P<0.01,P<0.05)。与模型组比较,中药组大鼠顶区皮质、额区皮质MOR mRNA及蛋白扩增产物电泳条带增强,顶区皮质MOR mRNA表达升高,顶区皮质和额区皮质MOR蛋白相对表达升高;下丘脑和海马MOR mRNA及蛋白电泳条带亮度减弱,下丘脑和海马MOR mRNA相对表达降低,海马MOR蛋白相对表达降低,差异均有统计学意义(P<0.01,P<0.05)。结论 MOR在PMS肝气逆证大鼠不同脑区呈现特异性表达,经前平颗粒表现出相应调节作用。

Objective To observe the effects of Jingqianping Granule （JG） on mRNA and protein expres- sions of p opioid receptor in the parietal cortex and the frontal cortex, the hypothalamus and hippocampus of pre- menstrual syndrome （PMS） Gan-qi invasion rats. Methods Twenty rats were selected to prepare the PMS Gan-qi invasion model. After modeling rats were divided into the model group and the Chinese herb treated group, ten in each group. Another 10 rats were selected as the normal control group. During the modeling, JG （1.6 g/kg） was given to rats in the Chinese herb treated group by gastrogavage, while equal volume of normal saline （1 mL/100 g） was given to rats in normal control group and the model group. All treatment was performed once daily for five suc- cessive days. The mRNA and protein expressions of IJ opioid receptor in the parietal cortex and the frontal cortex, the hypothalamus and hippocampus were detected using RT-PCR and Western blot respectively. Results Com- pared with the normal control group, the bands of products of MOR mRNA and protein in the parietal cortex and the frontal cortex were relatively weaker in the model group, and the optical density value decreased. The MOR mRNA and protein expressions in the parietal cortex and the frontal cortex relatively decreased. But the bands of products of MOR mRNA and protein in the hypothalamus and hippocampus were relatively stronger and optic value in- creased. The MOR mRNA and protein expressions in the hypothalamus and hippocampus relatively increased with statistical difference （P 〈0. 01, P 〈0. 05）. Compared with the model group, the bands of products of MOR mRNA and protein in the parietal cortex and the frontal cortex were relatively enhanced, the MOR mRNA expression in the parietal cortex increased, the MOR protein expression in the parietal cortex and the frontal cortex increased in the Chinese herb treated group. The bands of products of MOR mRNA and protein in the hypothalamus and hippocam- pus were relatively weaker. The MOR mRNA and protein expressions in the hypothalamus and hippocampus rela- tively decreased. The MOR protein expression in the hippocampus decreased relatively with statistical difference （P〈0. 01, P〈0.05）. Conclusions Expression of ~ opioid receptor in brains of PMS Gan-qi invasion rats was re- gionally specific. Administration of JG showed corresponding regulatory effects.