黄连木雌雄株叶水溶性酚类物质和两种氧化酶活性的比较

Comparative Analysis of Water-soluble Phenolic Substances and Oxidases Activity in the Male and Female Plant of Pistacia chinensis

黄连木为雌雄异株植物。为探索鉴别黄连木幼苗性别的方法,应用比色法对其雌雄株不同发育阶段叶片中水溶性酚类物质的含量、过氧化物酶(POD)活性和多酚氧化酶(PPO)相对活性进行测定和比较分析,结果表明:不同性别的黄连木叶片水溶性酚类物质含量存在极显著的差异(p<0.01);POD、PPO相对活性在雌雄株间也存在显著差异,特别在老叶中则存在极显著差异,认为黄连木雌雄株老叶中的水溶性酚类物质含量差异,可以作为其性别鉴定的依据,为进一步研究黄连木植株早期性别鉴定提供参考。

The Pistaeia chinensis plant is dioecious. There is a need to identify the sapling sex for reforestation practices. In order to explore an easy method for sex identification ofPistaeia chinensis plant, this study tested the phenolic substances, the peroxidase （POD） activity and polyphenol oxidase （PPO） activity in female and male tree leaves by colorimetric method. The differences in the content of the phenolic substances and the activities of two oxidases between female and male plants were analyzed through t-test. The results showed that there was a significant difference （p〈0.01） in the amount of water-soluble phenolic substances in leaves between the male and female plants. The activities of POD and PPO in male and female were significantly different （p〈0.05）, especially in old leaves （p〈0.01）. It was suggested that the sex ofPistacia chinensis seedlings might be identified through the comparison of the water-soluble phenolic substances content in old leaves. The paper provided the scientific basis for further research on sex determination ofPistaeia chinensis plants.