钝顶螺旋藻藻蓝蛋白抗百草枯诱导大鼠肺纤维化的研究

Experimental study on the therapeutic effect of C-phycocyanin against pulmonary fibrosis induced by paraquat in rats

目的探讨钝顶螺旋藻（spirulina platensis）藻蓝蛋白（C-phycocyanin，C—PC）对百草枯（paraquat，PQ）染毒诱导大鼠肺纤维化的治疗作用。方法选取健康Wistar大鼠90只，随机分为正常对照组、模型组（PQ组）和藻蓝蛋白治疗组（C—PC组），每组30只。PQ组、C—PC组以PQ（50mg／kg）一次灌胃染毒造模。造模后，正常对照组、PQ组大鼠每天给予生理盐水（1m/100g）灌胃，C—PC组每天给予C—PC（50mg／kg）灌胃，并在第1、3、7、14、28天各组随机取6只大鼠右肺下叶肺组织匀浆测羟脯氨酸（HYP）、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力；取左肺部分组织行HE染色、Masson染色进行病理观察，并用免疫组化方法观察肺组织中转化生长因子-β1（TGF-β1）、核因子-κB亚基p65（NF—κB p65）及肿瘤坏死因子-α（TNF-α）的表达。结果各观察时点C—PC组大鼠肺组织中HYP和14、28d MDA含量明硅低于PQ组，差异有统计学意义（P〈0．05，P〈0．01）。1、7、14、28d时C—PC组大鼠肺组织中SOD活力明显高于PQ组，差异有统计学意义（P〈0．05，P〈0．01）。PQ组和C—PC组肺组织中TGF-β1的表达及NF—κB p65和TNF-α表达明显高于正常对照组，C—PC组肺组织的TGF-β1表达及NF-κB p65和TNF-α表达明显低于PQ组，差异均有统计学意义（P〈0．05，P〈0．01）。病理学观察显示C—PC能减轻PQ染毒大鼠肺泡炎和肺纤维化程度。结论C—PC对PQ诱导的大鼠肺泡炎及肺纤维化有抑制作用。

Objective To investigate the therapeutic effect of C-phycocyanin （C-PC） from Spirulina platensis on paraquat （PQ）-induced pulmonary fibrosis in rats. Methods A total of 90 healthy Wistar rats were randomly and equally divided into normal control group, model group （PQ group）, and C-PC treatment group （C-PC group）. Each rat in the PQ group and C-PC group were orally administered with a single dose of PQ （50 mg/kg） to establish a rat model of PQ poisoning. Then, the rats in the normal control group and PQ group were orally given saline solution （ 1 ml/100 g） every day, and the rats in the C-PC group were orally given C-PC （50 mg/kg） every day. Six rats were randomly selected from each group on days 1, 3, 7, 14, and 28. The inferior lobe of each rat＇s right lung was homogenized for the measurement of hydroxyproline （HYP） and maleic dialdehyde （MDA） levels and superoxide dismutase （SOD） activity. Parts of each rat＇s left lung were subject to HE staining and Masson staining for pathological observation, and the expression of transforming growth factor-β1 （TGF-β1）, nuclear faetor-kappa B p65 （NF-κB p65）, and tumor necrosis factor-or （TNF-α） in lung tissue was measured by immunohistoehemistry. Results The HYP levels on days 1, 3, 7, 14, and 28 and MDA levels on days 14 and 28 were significantly lower in the C-PC group than in the PQ group （P〈0.05, P〈0.01）. The SOD activity was significantly higher in the C-PC group than in the PQ group on days 1, 7, 14, and 28 （P〈0.05, P〈 0.01 ）. The protein content of TGF-β1 and the activities of NF-κB p65 and TNF-α in the PQ group and C-PC group were significantly higher than those in the normal control group, while the indices in the C-PC group were significantly lower than those in the PQ group （P〈0.05, P〈0.01 ）. The pathological observation showed that C- PC could alleviate pulmonary alveolitis and fibrosis in rats with PQ poisoning. Conclusion C-PC can significantly inhibit PQ-indueed pulmonary alveolitis and fibrosis in rats.