硼卡钠对辐射损伤细胞增殖能力的影响

Effects of BSH on the Proliferation of Cells Irradiated by γ-Ray

目的研究硼卡钠(sodium borocaptate,BSH)对Hela和HIEC细胞辐射后增殖能力的影响。方法用137Csγ射线对BSH作用下的Hela和HIEC细胞进行不同剂量的一次性照射(1Gy,2Gy,4Gy,6Gy,8Gy),剂量率为0.8Gy/min。分别采用四甲基偶氮噻唑蓝(MTT)法检测和细胞克隆形成法检测细胞增殖情况。结果 MTT实验表明0.0036mg/ml～0.1150mg/ml BSH对3Gy照射后细胞具有明显的辐射保护作用(P<0.05);克隆形成实验发现BSH终浓度为0.060mg/ml时,对不同剂量的辐射损伤具有不同程度的保护作用。结论一定浓度的BSH对照后Hela细胞和HIEC细胞具有明显的促进增殖作用。

Objective To study the protective effect of BSH on the proliferation of Hela and HIEC cells irradiated by γ - ray. Methods Hela and HIEC cells which were given BSH ,were exposed to 137Cs 7 - ray one time with the different doses （ 1, 2, 3, 4, 6, 8 Gy） at the dose rate of 0.8 Gy/rain. The proliferation of cells were measured by MTT assay and cell clone formation assay. Results MTT assay showed that BSH has a significant protective effect on cells at the concentration of 0. 0036 mg/ml to 0.1150 mg/ml. Clone formation assay showed that the protective effect of BSH （0.060mg/ml） was different when cells exposed to different doses. Conclusion BSH can increase proliferation of Hela and HIEC cells irradiated by γ- ray.