免疫亲和色谱-高效液相色谱法测定河水和土壤中克百威、三唑磷和绿磺隆残留量

IAC-HPLC Determination of Residual Amounts of Carbofuran,Triaophos and Chlorsulfuran in River Water and Soil

将河水或土壤样品的丙酮提取液通过串联的分别装有克百威、三唑磷和绿磺隆免疫亲和吸着剂的3支免疫亲和色谱柱(IAC)或同时装有上述3种吸着剂的多抗体IAC,对样品中克百威、三唑磷及绿磺隆3种农药进行分离。采用串联IAC分离时,在样品溶液过柱后,将串联的IAC柱分开,并用不同配比的甲醇-水溶液分别从3支IAC柱上洗脱农药,用ELISA和HPLC分别测得农药的含量。采用多抗体IAC分离时,用甲醇-水溶液洗脱后,用上述两种方法测得洗脱液中3种农药的含量。对2种分离方法作回收和精密度试验,两方法的回收率和重复性基本一致。

Sample solution of river water or acetone extract of soil sample was passed through either a set of 3 immunoaffinity chromatographic columns （IAC） connected in series in which immunoaffinity sorbents of carbofuran, triaophos and ehlorsulfuran were filled separately, or through a multi-antibody IAC in which the 3 immunoaffinity sorbents were filled together, to separate the 3 pesticides, carbofuran, triaophos and chlorsulfuran, from the sample solution. When the IAC in series was used in separation, the 3 columns were disconnected after passing-through of the sample solution, and each of the columns was eluted with mixtures of CHaOH and H20 （mixed in different ratios）. Amounts of carbofuran, triaophos and chlorsulfuran were determined by ELISA and HPLC separately in the respective eluates from the columns. When the multi-antibody IAC was used in separation, the 3 pesticides in the eluate were determined simultaneously by ELISA and HPLC separately. Tests for recovery and precision for the 2 methods of separation were made, giving consistent results of recovery and repeatability between the 2 methods.