摘要
目的探讨大鼠骨骼肌肌动蛋白纤维的形态学变化与死亡时间的相关性。方法分别采用激光扫描共聚焦显微镜和透射电镜对大鼠死后不同时间骨骼肌(右后肢的内收大肌)肌动蛋白纤维的形态学变化进行观察。结果大鼠死后,透射电镜观察可见由肌动蛋白构成的细肌丝逐渐崩解、紊乱,直至肌小节和细肌丝结构消失;激光扫描共聚焦显微镜观察可见,自死后24h起,骨骼肌纤维横纹可有小片状或弥漫状抗肌动蛋白抗体缺染灶出现,并且抗肌动蛋白抗体染色面积随着死亡时间的延长而逐渐减少,其变化趋势与死亡时间相关(Y=0.934-0.005X,R2=0.95,P<0.05);大鼠死后168h内,肌动蛋白阳性产物的积分光密度值随死亡时间的延长而逐渐降低,组间差异均具有统计学意义(P<0.05),至死后168h,几乎无抗肌动蛋白抗体染色。结论大鼠骨骼肌肌动蛋白纤维的形态学改变与死亡时间具有相关性。
Objective To investigate the morphologic changes of actin in skeletal muscles of rats associated with postmortem interval (PMI). Methods The morphologic changes of actin filament in the skeletal muscles (adductor magnus of right hind leg) of 28 rats were observed by laser scanning confocal microscope (LSCM) and transmission electron microscope (TEM) at different postmortem intervals (0, 24, 48, 72, 96, 120 and 168 h). Results The TEM revealed that actin filament began to disintegrate with the lapse of PMI, and eventually the structure of sarcomere and actin filament disappeared. The LSCM showed depletion of antbactin antibody staining in the skeletal muscles and the extent of staining decreased with extension of PMI correlatively (Y= 0. 934-0. 005X, R^2=0.95,P=0. 05). The content of actin positive products reduced with PMI (P〈0. 05). No actin positive products were detected 168 h after death. Conclusion The morphologic changes of actin filament in skeletal muscles may become a useful indicator for the estimation of death time.
出处
《四川大学学报(医学版)》
CAS
CSCD
北大核心
2012年第5期694-696,共3页
Journal of Sichuan University(Medical Sciences)
关键词
肌动蛋白
激光扫描共聚焦显微镜透射电镜病理学
Actin
Laser scanning confocal microscope
Transmission electron microscopePathology