线粒体抗体芯片法对人肝脏代谢蛋白的发育分析(英文)

Developmental analysis of liver metabolic proteins using mitochondrial antibody microarrays

目的分析比较代谢相关蛋白在成人和胎儿肝脏中的分布及其丰度。方法使用含19个线粒体单抗的蛋白芯片分析4种肝组织总蛋白样本(成人肝组织匀浆蛋白,胎肝匀浆蛋白,成人肝线粒体蛋白和胎肝线粒体蛋白),通过生物信息学工具对蛋白表达丰度进行比较,探讨蛋白质代谢相关的途径。结果醛氧化酶和羰基还原酶在成人肝线粒体中的表达较胎儿分别上调2.6和1.7倍。皮质类固醇11-β-脱氢酶同工酶1,环氧化物水解酶1和纤维蛋白原β链蛋白则分别下调1.7,1.9和2.2倍。环氧水解酶1和谷胱甘肽转移ω-1在成人和胎儿肝匀浆样本中存在显著差异。结论肝脏代谢相关蛋白的表达在成人肝脏和胚胎肝脏中存在显著的差别。该研究将有助于更好地理解代谢蛋白的发生和发展,并确定肝脏代谢标志物。

Objective To investigate the abundance of metabolic proteins in adult and fetal human livers. Methods Adult liver homogenate proteins, fetal liver homogenate proteins, adult liver mitochondrial proteins and fetal liver mitochondrial proteins were obtained from fetal or adult liver tissues and examined using the antibody microarrays containing 19 liver monoclonal mitochondrial antibodies. The protein expression abundances were compared among the 4 protein fractions and the pathways related to protein metabolisms were explored. Results In adult liver mitochondria, aldehyde oxidase and carbonyl reductase were up-regulated by 2.6 and 1.7 folds, respectively, whereas corticosteroid 11-beta-dehydrogenase isozyme 1, epoxide hydrolase 1 and fibrinogen beta chain protein were down-regulated by 1.7, 1.9 and 2.2 folds, respectively compared to those in fetal liver mitochondria. The abundance of epoxide hydrolase 1 and glutathione transferase omega-1 was significantly different between adult and fetal liver homogenate samples. Conclusion Our results demonstrate a dear difference in the expression profiles of metabolic proteins in the liver between adults and human fetuses to allow a better understanding of the occurrence and development of the metabolic proteins and the identification of markers of liver metabolism.