不同细胞促进脂肪移植存活的实验研究

Effects of different human adipose-derived cells in promoting human adipose tissue engraftment in nude mice

目的探讨应用自人脂肪组织来源的不同细胞辅助脂肪移植,寻找促进移植物存活率的最佳种子细胞的有效方法,为干细胞进一步运用于临床提供实验依据。方法从临床抽脂病人获取脂肪组织并提炼细胞,将0.3 ml待移植的脂肪颗粒分别与以下细胞进行混合处理:(1)低氧脂肪来源间充质干细胞(A组);(2)脂肪来源间充质干细胞(B组);(3)血管基质层细胞(SVFs)(C组);(4)加完全培养基的单纯脂肪颗粒为对照组(D组)脂肪颗粒与相应细胞混合后,注射移植于6只裸鼠背部皮下。术后3个月观察移植物情况,通过组织学、HE染色等方法进行分析。结果 A^D组湿重分别为(61.67±8.165)、(91.67±1.472)、(96.67±5.164)和(40.83±4.916)mg,A、B、C组脂肪存活率均高于D组(P<0.05),B、C两组之间比较差异无统计学意义(P>0.05)且都高于A组。A、B、C组血管密度均高于组D,且C组明显高于其他3组(P<0.05)。A、B、C组存活脂肪细胞计数均高于D组,且B、C组最高(P<0.05),纤维组织计数均低于D组(P<0.05)。结论来源于人自体干细胞复合脂肪颗粒能够显著提高移植脂肪组织的成活率,其中血管基质层细胞及脂肪来源间充质干细胞移植脂肪的存活率最高。

Objective To explore the optimal seed ceils derived from human adipose tissue for promoting the engraftment of transplanted adipose tissue in nude mice. Methods Human adipose tissue granules （0.3 ml） obtained from patients undergoing liposuction were mixed with hypoxic adipose-derived stem ceils （ADCs, group A）, ADCs （Group B）, stromal vascular fraction （SVF） cells （group C）, or pure adipose tissue granules in complete culture medium particles （group D）. The mixtures were injected subcutaneously on the back of 6 nude mice, and the transplanted adipose tissues were harvested 3 months later to examine the engraftment using histological method and HE staining. Results The wet weights of the adipose grafts in groups B and C （91.67±1.472 mg and 96.67±5.164 mg, respectively） were similar （P〉0.05）, but both significantly higher than those in groups A and D （61.67±8.165 mg and 40.83 ±4.916 mg, respectively, P〈0.05）. The grafts in groups A, B and Cshowed a significantly higher blood vessel density than those in group D; the blood vessel density was the highest in group C （P〈0.05） and similar in groups A and B （P〉0.05）. Histologically, the adipose grafts in groups B and C consisted predominantly of adipose tissue, with less necrosis and fibrosis than those in groups A and D （P〈0.05）. The fibrosis count was the highest in group D （P〈0.05）, and similar in groups B and C （/2〉0.05）. Conclusion The adipose-derived stem cells, especially ASCs and SVFs, can promote the engraftment of human adipose tissue in nude mice, indicating their potential clinical value in adipose tissue transplantation.