APPswe/PS△E9双转基因小鼠脑组织miRNA的表达

Expression profile of miRNAs in APP swe/PS△E9 transgenic mice

目的观察APPswe/PS△E9双转基因小鼠与正常小鼠脑组织内miRNA的差异表达,探索miRNA在阿尔茨海默病中的可能作用。方法采用6月龄APPswe/PS△E9双转基因小鼠作为实验组,同月龄、同种系野生型小鼠C57作为对照组,基因芯片检测两组小鼠脑组织miRNA表达;比较两组小鼠miRNA的差异表达。结果与对照组比较,实验组中表达上/下调2倍以上的miRNA有miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p、miR-669-3p、miR-142-5p、miR-144、miR-466f-3p、miR-466g、miR-200a、miR-200b、miR-96等12种,但有统计学意义(P<0.05)的均是下调的5种miRNA:miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p和miR-669-3p。结论 miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p和miR-669-3p可能是在APPswe/PS△E9双转基因小鼠发病中有意义的miRNA。

Objective To observe the changes of miRNA expression profiles in APPswe/PS△ E9 transgenic mice and explore the possible roles of miRNA in the pathogenesis of Alzheimer＇ s disease. Methods Using miRNA chip technique, we examined the miRNA expression in the brain tissue of 6-month-old APPswe/PS A E9 transgenic mice, with age-matched wild-type mice as the control group. Results Twelve miRNAs showed differential expressions by more than two folds in APPswe/PS A E9 transgenic mice, namely miRNA-135a, miRNA-135a-2＊, miRNA-298, miRNA-466b-3p, miR-669-3p, miR-142-5p, miR-144, miR-466f-3p, miR-466g, miR-200a, miR-200b and miR-96. Five miRNAs were significantly down-regulated in the transgenic mice, including miRNA-135a, miRNA-135a-2＊, miRNA-298, miRNA-466b-3p, and miR-669-3p. Conclusion The 5 down- regulated miRNA may play important roles in the pathogenesis of AD in APPswe/PS A E9 transgenic mice.