微管相关蛋白2和巢蛋白在人胚胎胃组织中的表达

Microtubule-associated protein 2 and nestin expressions in human embryonic and fetal gastric tissues

目的探讨微管相关蛋白2(MAP-2)和巢蛋白(Nestin)与人胚胎胃组织的发育关系。方法应用免疫组织化学PV法检测第2、3、4三个月龄段,人胚胎胃壁、贲门和幽门组织内MAP-2和Nestin的表达规律。结果第2、3、4三个月龄段,MAP-2和Nestin阳性表达主要集中于人胚胎胃壁、贲门和幽门组织黏膜下神经丛和肌间神经丛,随胎龄的增大,MAP-2和Nestin蛋白在肌间神经丛内阳性表达细胞数量和强度均呈增高趋势。而MAP-2和Nestin蛋白在胃壁、贲门和幽门组织的黏膜上皮和腺体处均呈阴性表达。结论 MAP-2和Nestin参与调节人胚胎胃组织的生长发育过程。

Objective To investigate the role of microtubule-associated protein 2 （MAP-2） and nestin in gastric development in human embryos and fetuses. Methods Immunohistochemistry was used to detect the expressions of MAP-2 and nestin proteins in the gastric cardia, pyloric and gastric tissues of human embryos and fetuses during the second, third and fourth month of development. Results In the second to fourth months of gestation, MAP-2 and nestin expressions were detected in the neural cells and neural fibers of the intermuscular nerve plexus and submucosal plexus in the gastric cardia, pyloric and gastric tissues. As the gestational age increased, the number of MAP-2- and nestin-positive ceils and the expression intensity all increased in the myenteric plexus, but MAP-2 and nestin expressions were negative in the glandular and mucosal tissues of human embryonic and fetal gastric cardia, pylorus or gastric wails. Conclusion MAP-2 and nestin participate in the regulation of the development of gastric tissues in human embryos.