空肠弯曲杆菌生物膜的体外形成及鉴定

Formation and identification of Campylobacter jejuni biofilm in vitro

采用结晶紫染色与正交试验相结合的方法,对空肠弯曲杆菌在不同温度、pH值、气体条件以及不同培养基条件下形成生物膜的能力进行了检测和比较;并以筛选出的最佳培养条件在盖玻片上形成的生物膜为对象,用光学显微镜及扫描电镜观察生物膜的结构。结果显示,在正常气体条件下培养1～3d,生物膜形成水平明显高于其他2种气体条件(P<0.01),4d后3种气体条件下的生物膜形成量均有增加,但3组间差异不显著,说明正常空气组分的条件更有利于生物膜的快速形成,而且37℃、正常空气条件下,在pH7.0的MH肉汤中培养5d,通过显微镜和扫描电镜观察,在盖玻片上可形成典型的、可观测的生物膜结构。

Crystal violet staining and orthogonal experiment were combined to detect the formation ability of biofilm of Campylobacter jejuni cultured in different temperatures,pH values,air conditions and media.The light microscope and scanning electron microscope were used to observe biofilm structure of C.jejuni that was cultured on the carrier to the coverslip in the optimal conditions.The results showed that the biofilm formation level in the normal air in one to three days was higher than that under the other two kinds of air conditions（microaerobic condition or 100 mL/L CO2 in air）（P0.01）.Thus in the last two days the biofilm formed more,but the differences among the three groups was not obvious.The result indicated that the normal air components conditions were more conducive to formation of biofilms.A typical biofilm structure of C.jejuni could be observed at 37 ℃ for five days,under the normal air conditions,in MH broth medium at pH=7.0.